Name: measuring post-stroke cerebral edema, infarct zone and blood-brain barrier breakdown in a single set of rodent brain samples
Uploaded: 2020-10-23T13:30:00
Description: Watch this Scientific Journal Video about Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples at JoVE.com

我们感谢马里娜·库舍里亚瓦、马克西姆·克里沃诺索夫、达里娜·亚库门科和叶夫根尼亚·贡查里克，他们支持我们的讨论，并为我们的讨论作出了有益的贡献。获得的数据是鲁斯兰·库茨博士论文的一部分。

下列程序是根据《赫尔辛基宣言》和《欧洲共同体实验动物使用准则》的建议进行的。内盖夫本古里安大学的动物护理委员会也批准了这些实验。
1. 为实验程序准备大鼠
<ol><li>选择成年雄性斯普拉格-道利大鼠，没有明示病理学，每只体重在300至350克之间。</li>
	<li>将所有大鼠在室温下保持在22°C，在实验前有12小时的光和暗周期。</li>
	<li>确保食物和水是可用的广告利比妥姆...

本议定书的主要目标是证明对缺血损伤的三个主要参数的一致测量：IZ、BE和BBB渗透性。先前在这一领域的研究表明，有可能在同一样本中一起执行其中一个或两个参数。除了这种由三部分组成的方法提供的成本降低外，它还提供了一个更理想的生物伦理模型，限制必须操作并随后实施安乐死的动物数量。与所有病理学技术一样，该方法受到无法动态观察缺血损伤的限制。
在?...

全世界发病率和死亡率最常见的原因之一是中风。在全球范围内，缺血性中风占所有中风病例的68%，而在美国缺血性中风占中风病例的87%。1，2。据估计，中风的经济负担达到340亿美元，在美国2和450亿欧元在欧盟3。动物中风模型是研究其病理生理学，开发新的评价方法，并提出新的治疗方案4。
缺血性中风发生在一个主要脑动脉的闭塞，通常是中脑动脉或其分支之一5。因此，缺血性中风的模型历来涉及中脑动脉闭塞（MCAO）6，7，8，9，10，11，12。继 MCAO 之后， 神经损伤最常通过测量梗塞区 （IZ） 来评估，使用 2，3，5 三苯甲酸酯氯化物 （TTC） 染色方法13，脑水肿 （BE） 使用 干燥或计算半球体积14，15，16，和血脑屏障（BBB）渗透性光谱技术使用埃文斯蓝色染色17，18，19。
传统的 MCAO 方法在三个大脑测量中每个都使用单独的大脑集。对于大量的样本量，这导致大量的安乐死动物，增加了伦理和财务考虑。减轻这些成本的替代方法将涉及在一组后 MCAO 啮齿动物大脑中测量所有三个参数。
先前曾尝试测量同一大脑样本中的参数组合。同时免疫荧光染色方法20 以及其他分子和生化分析21 已描述后TTC染色在同一大脑样本。我们以前计算过脑半球的体积来评估脑水肿，并进行了TTC染色，以计算同一大脑集15的梗塞区。
在本协议中，我们提出了一种经过改进的 MCAO 技术，通过确定同一组啮齿动物大脑中的 IZ、BE 和 BBB 渗透性来测量缺血性脑损伤。IZ通过TTC染色测量，BE通过计算半球体积来测量，BBB渗透性通过光谱方法19获得。在此协议中，我们使用了经过修改的 MCAO 模型，基于将单滤导管直接插入和固定到内部胡萝卜动脉 （ICA） 和进一步阻断流向中脑动脉 （MCA）22的血液。与传统的MCAO方法16、22相比，这种修改后的方法表明死亡率和发病率有所下降。
这种新方法为测量 MCAO 之后的神经损伤提供了财务健全和道德模型。这种对缺血性脑损伤主要参数的评估将有助于全面研究其病理生理学。

<table>
	<tbody>
		<tr>
			<td>2 mL Syringe</td>
			<td>Braun</td>
			<td>4606027V</td>
			<td></td>
		</tr>
		<tr>
			<td>2% chlorhexidine in 70% alcohol solution</td>
			<td>Sigma-Aldrich</td>
			<td>500 cc</td>
			<td>Provides general antisepsis of the skin in the operatory field</td>
		</tr>
		<tr>
			<td>27 G Needle with Syringe</td>
			<td>Braun</td>
			<td>305620</td>
			<td></td>
		</tr>
		<tr>
			<td>3-0 Silk sutures</td>
			<td>Henry Schein</td>
			<td>1007842</td>
			<td></td>
		</tr>
		<tr>
			<td>4-0 Nylon suture</td>
			<td>4-00</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Brain &#38; Tissue Matrices</td>
			<td>Sigma-Aldrich</td>
			<td>15013</td>
			<td></td>
		</tr>
		<tr>
			<td>Cannula Venflon 22 G</td>
			<td>KD-FIX</td>
			<td>183603985447</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge Sigma 2-16P</td>
			<td>Sigma-Aldrich</td>
			<td>Sigma 2-16P</td>
			<td></td>
		</tr>
		<tr>
			<td>Compact Analytical Balances</td>
			<td>Sigma-Aldrich</td>
			<td>HR-AZ/HR-A</td>
			<td></td>
		</tr>
		<tr>
			<td>Digital weighing scale</td>
			<td>Sigma-Aldrich</td>
			<td>Rs 4,000</td>
			<td></td>
		</tr>
		<tr>
			<td>Dissecting scissors</td>
			<td>Sigma-Aldrich</td>
			<td>Z265969</td>
			<td></td>
		</tr>
		<tr>
			<td>Eppendorf pipette</td>
			<td>Sigma-Aldrich</td>
			<td>Z683884</td>
			<td></td>
		</tr>
		<tr>
			<td>Eppendorf tube</td>
			<td>Sigma-Aldrich</td>
			<td>EP0030119460</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorescence detector</td>
			<td>Tecan, M&#228;nnedorf Switzerland</td>
			<td>Model: Infinite 200 PRO multimode reader</td>
			<td>Optional.</td>
		</tr>
		<tr>
			<td>Fluorescence detector</td>
			<td>Molecular Devices LLC</td>
			<td>VWR cat. # 10822 512 SpectraMax Paradigm Multi Mode Microplate Reader Base Instrument</td>
			<td>Optional.</td>
		</tr>
		<tr>
			<td>Gauze sponges</td>
			<td>Fisher</td>
			<td>22-362-178</td>
			<td></td>
		</tr>
		<tr>
			<td>Heater with thermometer</td>
			<td>Heatingpad-1</td>
			<td>Model: HEATINGPAD-1/2</td>
			<td></td>
		</tr>
		<tr>
			<td>Hemostatic microclips</td>
			<td>Sigma-Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Horizon-XL</td>
			<td>Mennen Medical Ltd</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Infusion cuff</td>
			<td>ABN</td>
			<td>IC-500</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro forceps</td>
			<td>Sigma-Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Micro scissors</td>
			<td>Sigma-Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Multiset</td>
			<td>Teva Medical</td>
			<td>998702</td>
			<td></td>
		</tr>
		<tr>
			<td>Olympus BX 40 microscope</td>
			<td>Olympus</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Operating forceps</td>
			<td>Sigma-Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Operating scissors</td>
			<td>Sigma-Aldrich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Optical scanner</td>
			<td>Canon</td>
			<td>Cano Scan 4200F</td>
			<td>Resolution 3200 x 6400 dpi</td>
		</tr>
		<tr>
			<td>Petri dishes</td>
			<td>Sigma-Aldrich</td>
			<td>P5606</td>
			<td></td>
		</tr>
		<tr>
			<td>Purina Chow</td>
			<td>Purina</td>
			<td>5001</td>
			<td>Rodent laboratory chow given to rats, mice and hamster is a life-cycle nutrition that has been used in biomedical research for over 5 decades. Provided to rats ad libitum in this experiment.</td>
		</tr>
		<tr>
			<td>Rat cages</td>
			<td>Techniplast</td>
			<td>2000P</td>
			<td>Conventional housing for rodents. Cages were used for housing rats throughout the experiment</td>
		</tr>
		<tr>
			<td>Scalpel blades #11</td>
			<td>Sigma-Aldrich</td>
			<td>S2771</td>
			<td></td>
		</tr>
		<tr>
			<td>Software</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Adobe Photoshop CS2 for Windows</td>
			<td>Adobe</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>ImageJ 1.37v</td>
			<td>NIH</td>
			<td></td>
			<td>The source code is freely available. The author, Wayne Rasband (wayne@codon.nih.gov), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA</td>
		</tr>
		<tr>
			<td>Office 365 ProPlus</td>
			<td>Microsoft</td>
			<td>-</td>
			<td>Microsoft Office Excel</td>
		</tr>
		<tr>
			<td>Windows 10</td>
			<td>Microsoft</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Reagents</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>2,3,5-Triphenyltetrazolium chloride</td>
			<td>Sigma-Aldrich</td>
			<td>298-96-4</td>
			<td></td>
		</tr>
		<tr>
			<td>50% trichloroacetic acid</td>
			<td>Sigma-Aldrich</td>
			<td>76-03-9</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol 96 %</td>
			<td>Romical</td>
			<td></td>
			<td>Flammable liquid</td>
		</tr>
		<tr>
			<td>Evans blue 2%</td>
			<td>Sigma-Aldrich</td>
			<td>314-13-6</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane, USP 100%</td>
			<td>Piramamal Critical Care, Inc</td>
			<td>NDC 66794-017</td>
			<td></td>
		</tr>
	</tbody>
</table>

measuring post-stroke cerebral edema, infarct zone and blood-brain barrier breakdown in a single set of rodent brain samples

全世界发病率和死亡率最常见的原因之一是缺血性中风。从历史上看，用于刺激缺血性中风的动物模型涉及中脑动脉闭塞（MCAO）。梗塞区、脑水肿和血脑屏障 （BBB） 故障被测量为反映 MCAO 后脑损伤程度的参数。这种方法的一个重大限制是，这些测量通常在不同的大鼠大脑样本中获得，由于大量大鼠需要安乐死以达到适当的样本大小，因此会导致伦理和经济负担。在这里，我们提出了一种方法，通过测量同一组大鼠大脑中的梗塞区、脑水肿和BBB渗透率，准确评估MCAO之后的脑损伤。这种新技术为评估中风的病理生理学提供了更有效的方法。

梗塞区测量
一项独立样本T测试表明，与16只假手术大鼠相比，19只接受永久性MCAO的老鼠的大脑梗塞体积显著增加（MCAO=7.49%，±3.57对。 香=0.31%±1.9，t（28.49）=7.56，p<0.01（见图2A）。 数据表示为反半球± SD 的平均百分比。
脑水肿测量
一项独立样本T测试表明，与16只假手术大...

Watch this Scientific Journal Video about Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples at JoVE.com

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

此协议描述了在同一组啮齿动物脑样本上测量缺血性脑损伤三个最重要的参数的新技术。仅使用一个大脑样本在道德和经济成本方面非常有利。

测量一组啮齿动物脑样本中的中风后脑水肿、梗塞区和血脑屏障破裂

One of the most common causes of morbidity and mortality worldwide is ischemic stroke. Historically, an animal model used to stimulate ischemic stroke ...

Research

JoVE Journal

Neuroscience

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

Although mice are the dominant model system for studying the genetic and molecular underpinnings of neuroscience, functional neuroimaging in mice remains ...

科研

神经科学

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

Disruption of blood-brain barrier (BBB) integrity is a common feature for different neurological and neurodegenerative diseases. Although the interplay ...

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测

Blood-brain barrier (BBB) is a specialized barrier that protects the brain microenvironment from toxins and pathogens in the circulation and maintains ...

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

一种体内评价大鼠缺血性中风模型中血脑屏障的破坏

Ischemic stroke leads to vasogenic cerebral edema and subsequent primary brain injury, which is mediated through destruction of the blood-brain barrier ...

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

爆炸性神经损伤对压生啮鼠中脑动脉的影响

Though there have been studies on the histopathological and behavioral effects of blast exposure, fewer have been dedicated to blast&#39;s cerebral ...

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

在鼠模型中使用聚焦超声波打开位置特定血脑屏障开口的台面方法

Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic ...

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

高通量图像引导立体定向神经导航和聚焦超声系统，用于在啮齿动物中打开血脑屏障

The blood-brain barrier (BBB) has been a major hurdle for the treatment of various brain diseases. Endothelial cells, connected by tight junctions, form a ...

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

聚焦超声诱导血脑屏障打开，用于靶向大脑结构和评估化学遗传神经调控

Acoustically Targeted Chemogenetics (ATAC) allows for the noninvasive control of specific neural circuits. ATAC achieves such control through a ...

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

通过跨内皮电阻检测对bEnd.3血管内皮细胞进行屏障功能完整性记录

The blood-brain barrier (BBB) is a dynamic physiological structure composed of microvascular endothelial cells, astrocytes, and pericytes. By coordinating ...

A 3D Blood-Brain Barrier Model Derived from Human Pluripotent Stem-Cells

Reconstruction of the Blood-Brain Barrier In Vitro to Model and Therapeutically Target Neurological Disease

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体外血脑屏障重建，模拟和治疗神经系统疾病

The blood-brain barrier (BBB) is a key physiological component of the central nervous system (CNS), maintaining nutrients, clearing waste, and protecting ...