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DOI :
10.3791/61345-v
•
8:00 min
June 4th, 2020
Chapters
0:05
Introduction
0:38
Cortex, Cerebellum, Brainstem, and Spinal Cord Dissection
2:51
Tissue Dissociation and Oligodendrocyte Precursor Cell Isolation
6:00
Results: OPC Differentiation in IFNγR-/- Mice
7:31
Conclusion
ここでは、マウスCNSからの複数のグリア細胞集団のin vitro単離のためのプロトコルを提示します。この方法では、局所ミクログリア、希突起膠細胞前駆細胞、およびアストロサイトの分離を可能にし、さまざまな培養システムにおけるそれぞれの表現型を研究することができます。
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