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DOI :
10.3791/61348-v
June 17th, 2020
Chapters
0:05
Introduction
1:13
Digestion of Ocular Tissue
3:34
Preparation of Cell Suspension
5:19
Staining of Cell Suspension for Mononuclear Phagocytes
6:49
Results: Mononuclear Phagocyte Populations in the Mouse Eye
9:26
Conclusion
본 프로토콜은 단핵세포, 마이크로글리아, 대식세포 및 수지상 세포를 포함하는 특정 안구 단핵 교핵 세포집단을 식별하기 위해 다중 파라미터 유동 세포측정 분석을 목적으로 전체 눈을 단일 세포 현탁액으로 소화하는 방법을 제공한다.
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