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DOI :
10.3791/61352-v
•
8:37 min
December 22nd, 2020
Chapters
0:04
Introduction
0:54
Animal Preparation
2:30
MRI‐Guided Targeting
4:35
Injection Solution Preparation
5:59
Insonation
7:10
Blood Brain Barrier (BBB) Opening Evaluation and Designer Receptors Activated Exclusively by Designer Drugs (DREADD) Stimulation
7:47
Results: BBB Opening and DREADD Expression Visualization
8:11
Conclusion
该协议描述了通过聚焦超声血脑屏障(BBB)开放进行基因传递所需的步骤,评估产生的基因表达以及通过组织学测试测量化学遗传受体的神经调控活性。
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