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DOI :
10.3791/61373-v
June 26th, 2020
Chapters
0:04
Introduction
0:38
Plasmid DNA Linearization
1:26
Full-Length Hepatitis E Virus (HEV) Genotype 3 p6 DNA In Vitro Transcription and RNA Purification
2:35
HepG2 Cell Preparation
3:47
HepG2 Cell Electroporation
5:40
Intra- and Extracellular Cell Culture Derived HEV (HEVcc) Particle Harvest
7:10
HepG2/C3A Cell Infection
8:47
Results: Representative High Titer HEV Stock Preparation and Characterization
9:46
Conclusion
ここで説明するE型肝炎ウイルス(HEV)の高ウイルス力価株を生成し、肝細胞を効率的に感染させる方法について説明する。提示された方法により、非エンベロープのウイルス粒子と同様に、様々な細胞株を接種するために収穫および使用することができる。
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