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DOI :
10.3791/61375-v
•
5:59 min
May 24th, 2020
Chapters
0:04
Introduction
0:51
Alignment of C. quinquefasciatus Pre-blastoderm Stage Embryos
1:46
Embryo Microinjection
3:32
Embryo Recovery and Hatching
4:08
Results: Survival and Mutation Rates of Embryos Injected with Single and Multiplexed gRNAs Targeting White
5:25
Conclusion
该协议描述了 Culex五角星胚胎的 微注射程序,这些胚胎通过CRISPR/Cas9基因编辑工具进行优化。该技术可以有效地生成特定地点、遗传的生殖系突变,可用于在这种研究不足的疾病载体中构建遗传技术。
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