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DOI :
10.3791/61398-v
May 16th, 2021
Chapters
0:04
Introduction
0:39
Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest
3:15
Insulin-Stimulated Glucose Uptake
4:18
Contraction-Stimulated Glucose Uptake
4:57
Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination
5:58
Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis
7:22
Conclusion
근육 포도당 흡수의 온전한 조절은 전신 포도당 항상성을 유지하는 데 중요합니다. 이 프로토콜은 전신 포도당 대사에 대한 다양한 생리적 개입의 영향을 묘사 할 때 단리되고 배양 된 성숙한 골격근에서 인슐린 및 수축 자극 포도당 흡수에 대한 평가를 제시합니다.
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