Differentiation of Brain Endothelial Cells from Human iPSCs
5:57
Transendothelial Electrical Resistance (TEER) as a Measure of Barrier Tightness
6:35
Preparation of Bacteria and Infection of iPSC-BECs
8:22
Results: Upregulation of Cytokines by iPSC-BECs upon Infection with Neisseria meningitidis
9:16
Conclusion
Transcript
This protocol demonstrates the usage of iPSC-derived brain-like endothelial cells to examine host pathogen interactions at the blood-brain barriers, such as the meningeal blood-CSF barriers, in a model that can better mimic brain endothelial cells
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Het hier beschreven protocol belicht de belangrijkste stappen in de differentiërende geïnduceerde pluripotente stamcel-afgeleide hersenachtige endotheelcellen, de voorbereiding van Neisseria meningitidis voor infectie en het verzamelen van monsters voor andere moleculaire analyses.