Differentiation of Brain Endothelial Cells from Human iPSCs
5:57
Transendothelial Electrical Resistance (TEER) as a Measure of Barrier Tightness
6:35
Preparation of Bacteria and Infection of iPSC-BECs
8:22
Results: Upregulation of Cytokines by iPSC-BECs upon Infection with Neisseria meningitidis
9:16
Conclusion
Transcript
This protocol demonstrates the usage of iPSC-derived brain-like endothelial cells to examine host pathogen interactions at the blood-brain barriers, such as the meningeal blood-CSF barriers, in a model that can better mimic brain endothelial cells
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여기에 설명된 프로토콜은 유도만능줄기세포 유래 뇌유사 내피세포의 분화, 감염을 위한 Neisseria meningitidis 준비 및 기타 분자 분석을 위한 샘플 수집의 주요 단계를 강조합니다.