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DOI :
10.3791/61401-v
•
8:33 min
March 11th, 2021
Chapters
0:04
Introduction
0:36
Unroofed Membrane Experiments
4:36
Patch-Clamp Fluorometry Experiment
5:56
Results: Representative Measurement of Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
7:55
Conclusion
该协议提出了一种在细胞环境中实时测量腺嘌呤核苷酸与受体结合的方法。结合测量为三硝基苯基核苷酸衍生物与用非规范荧光氨基酸标记的蛋白质之间的Förster共振能量转移(FRET)。
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