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DOI :
10.3791/61401-v
•
8:33 min
March 11th, 2021
Chapters
0:04
Introduction
0:36
Unroofed Membrane Experiments
4:36
Patch-Clamp Fluorometry Experiment
5:56
Results: Representative Measurement of Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
7:55
Conclusion
このプロトコルは、細胞環境において受容体に結合するアデニンヌクレオチドをリアルタイムで測定する方法を提示する。結合は、トリニトロフェニルヌクレオチド誘導体と非標準的な蛍光アミノ酸で標識されたタンパク質との間のフェルスター共鳴エネルギー移動(FRET)として測定されます。
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