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DOI :
10.3791/61440-v
•
10:22 min
June 30th, 2020
Chapters
0:04
Introduction
1:03
Plant Material Preparation
1:57
Ascorbic Acid (AA) Standard Preparation
2:34
AA and Dehydroascorbic Acid (DHAA) Extraction
3:40
DHAA Reduction for Total AA (TAA) Extraction
4:31
Ultra-High-Performance Liquid Chromatography-Ultraviolet Preparation
6:46
AA Determination
7:21
AA and DHAA Quantification
8:18
Results: Representative Vitamin C Quantification in Lactuca Species
9:44
Conclusion
我们提出了一种快速可靠的方法来量化维生素C在 Lactuca spp.使用 UPLC-UV,有可能转移到其他植物。关键步骤是样品制备和维生素C提取在稳定条件下,减少脱氢抗坏血酸抗坏血酸和优化色谱过程。
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