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DOI :
10.3791/61446-v
May 15th, 2020
Chapters
0:04
Introduction
0:58
EB, NPC, and Neuron Differentiation
3:00
Fixation and Embedding
4:24
Immunofluorescence
5:45
Results: E14 Differentiation into EBs, NPCs, and Neurons
7:41
Conclusion
우리는 매달려 드롭 방법을 사용하여 신경 세포로 마우스 배아 줄기 세포의 체외 분화에 대한 절차를 설명합니다. 또한 RT-qPCR, 면역형광, RNA-seq 및 유동 세포종을 통해 포괄적인 현상 분석을 수행합니다.
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