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DOI :
10.3791/61453-v
•
8:51 min
May 5th, 2021
Chapters
0:04
Introduction
0:28
Large-Scale Culture Plate (LSCP) Gravid Adult Spot Bleaching
1:21
LSCP Sample Harvest
3:02
Population Size Estimation
4:19
Large Particle Flow Cytometry (LPFC) Preparation
5:27
Population Distribution Documentation and 384-Well Imaging Plate Preparation
6:24
Results: Representative Large-Scale Mixed-Stage C. elegans Population Evaluation
8:06
Conclusion
オミックス研究でカエノハブディティス・エレガンス(C.エレガンス)を使用するには、比較分析のためにプラットフォーム間で1つのサンプルを測定できるワームの集団を大量に生成する方法が必要です。ここでは、大規模な培養プレート(LSP)上でC.エレガン群集団を培養し、人口増加を記録する方法を紹介する。
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