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DOI :
10.3791/61470-v
•
9:08 min
October 14th, 2021
Chapters
0:05
Introduction
1:17
Peripheral Blood Mononuclear Cell (PBMC) Isolation
3:42
CD4+CD45RO+ T Cell Purification
5:18
Chemical Activation
7:00
Results: Representative CD4+CD45RO+ T Cell Purity and Gene Expression Analysis
8:01
Conclusion
我们提出了一种简单的方案,用于从诊断为Sézary综合征的患者获得的全血中分离外周血单核细胞,然后选择CD4 + T细胞,用phorbol12-肉豆蔻酸酯13-乙酸盐和A23187离子载体刺激它们,并制备RNA进行转录组学分析。
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