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DOI :
10.3791/61471-v
•
7:00 min
June 24th, 2020
Chapters
0:04
Introduction
0:50
Preparation for Tissue Dissection
1:36
Zebrafish Brain Dissection and Single Nuclei Isolation
3:59
FACS Based Nuclei Enrichment
5:16
Results: Single Nuclei Isolation and Enrichment
6:04
Conclusion
单核分离依赖于细胞膜的分离和基于洗涤剂的渗透,这些步骤需要优化,并且容易引入技术伪影。我们演示了一种洗涤剂和无酶协议,用于直接从整个组织中快速分离完整的核,产生适合单核RNA-seq(snRNA-Seq)或 ATAC-seq 的核。
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