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DOI :
10.3791/61471-v
•
7:00 min
June 24th, 2020
Chapters
0:04
Introduction
0:50
Preparation for Tissue Dissection
1:36
Zebrafish Brain Dissection and Single Nuclei Isolation
3:59
FACS Based Nuclei Enrichment
5:16
Results: Single Nuclei Isolation and Enrichment
6:04
Conclusion
単核単離は、細胞膜の解離および洗剤ベースの透過性、最適化を必要とし、技術的なアーティファクトを導入する傾向があるステップに依存しています。我々は、単核RNA-seq(snRNA-Seq)またはATAC-seqに適した核を得て、組織全体から直接無傷核を迅速に単離するための洗浄剤および酵素フリープロトコルを実証する。
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