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DOI :
10.3791/61489-v
June 25th, 2020
Chapters
0:04
Introduction
0:33
Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
1:10
IMAC Stage Tip Phosphopeptide Enrichment
2:44
Hp-RP C18 Stage Tip Phosphopeptide Fractionation
3:37
Results: Representative Osmotic Stress Signaling in Arabidopsis
5:05
Conclusion
ここに提示されるホスホプロテオミクスアプローチ、すなわち、シロイヌモゼスホスファプロテオームのハイスループットと深いカバレッジを提供するリンポプロテオミクスに基づく抽出チップを停止して行く。このアプローチは、シロイヌナズナの浸透性ストレスシグナル伝達の概要を示す。
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