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DOI :
10.3791/61535-v
July 8th, 2020
Chapters
0:04
Introduction
0:43
Obtaining Kidney Samples from HIGA Mice
1:27
Total RNA Purification from Kidney Samples
2:14
cDNA Synthesis and qRT‐PCR of miRNA
3:55
Results: Expression Levels of miRNAs in the Kidneys of HIGA Mice
4:57
Conclusion
マイクロRNAはIgA腎症の病態形成に関与しています。我々は、IgA腎症モデルマウス(HIGAマウス)の腎臓におけるマイクロRNA発現レベルを確実に検出する方法を開発しました。この新しい方法は、IgA腎症におけるmiRNAの関与をチェックすることを容易にします。
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