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DOI :
10.3791/61599-v
•
11:40 min
January 21st, 2021
Chapters
0:05
Introduction
1:02
Tissue Slice Preparation
4:04
Microinjection
7:05
Tissue Culture and Tissue Slice Processing for Immunofluorescence
9:39
Results: Robotic Microinjection into Apical Progenitors and Neurons
10:57
Conclusion
이 프로토콜은 단일 신경 줄기 세포와 뇌 슬라이스의 뉴런으로 미세 주입을위한 로봇 플랫폼의 사용을 보여줍니다. 이 기술은 다재다능하며 높은 공간 해상도로 조직에서 세포를 추적하는 방법을 제공합니다.
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