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DOI :
10.3791/61602-v
August 25th, 2020
Chapters
0:04
Introduction
0:41
Fluorescent Tag Insertion into the Chromosomal Genome of P. aeruginosa
3:02
Preparation of Agarose Pad
3:57
Imaging with a Two-photon Microscopy Setup
6:44
Results: FLIM-FRET Data Visualization and Analysis
8:57
Conclusion
我们在此描述一个协议,使用FLIM-FRET测量来描述活 伪多 西诺萨中两种高度不同的表达蛋白之间的蛋白质-蛋白质相互作用。该协议包括细菌菌株结构、细菌固定、成像和成像后数据分析程序。
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