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DOI :
10.3791/61603-v
March 5th, 2021
Chapters
0:05
Introduction
0:48
Microscope Slide Preparation
2:06
Single Particle Darkfield Microscopy (DFM) Tracking
2:53
Data Acquisition
4:21
Red/Green Values
4:59
Data Analysis
5:30
Mean Square Displacement (MSD), Multi-Particle, and Time Analyses
6:33
Results: Representative Single Nanoparticle DFM of AuNR Diffusion Dynamics
8:39
Conclusion
ここでは、従来の暗視野顕微鏡を用いて、細胞膜上の金ナノロッド(AuNRs)のダイナミクスを監視する方法を示す。単一の AuNR の位置と向きは ImageJ および MATLAB を使用して検出され、AuNRs の拡散状態は単一の粒子追跡解析によって特徴付けられる。
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