Name: op-ivm: combining in vitro maturation after oocyte retrieval with gynecological surgery
Uploaded: 2021-05-09T14:00:00
Description: Watch this Scientific Journal Video about OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery at JoVE.com

本研究由国家重点研发计划（编号：2017YFC1002000、2018YFC1004001、2019YFA0801400）、国家自然科学基金（编号：81571386、81730038）、CAMS医学创新基金（2019-I2M-5-001）和中国首都健康发展专项（2018-2-4095）资助。

注：与OP-IVM方法相关的研究已获得北京大学第三医院机构审查委员会（IRB）和北京大学伦理委员会的批准（2014S2004）。OP-IVM的摘要如图 1所示。分步过程将在下一节中介绍。
1. 向合适的患者介绍OP-IVM
<ol><li>确定可能受益于OP-IVM的潜在患者，例如步骤1.1.1-1.1.3中描述的患者：<ol>
<li>多囊卵巢综合征 （PCOS） 有克罗米芬耐药的患者，需要腹腔镜卵巢钻孔手?...

<ol>
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C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+in+vitro+maturation+techniques+for+clinical+applications.">Development of in vitro maturation techniques for clinical applications.</a> Fertility and Sterility. 108 (4), 577-584 (2017).</li><li>Hatirnaz, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oocyte+in+vitro+maturation:+A+sytematic+review.">Oocyte in vitro maturation: A sytematic review.</a> Turkish Journal of Obstetrics and Gynecology. 15 (2), 112-125 (2018).</li><li>Gremeau, A. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+maturation+or+in+vitro+fertilization+for+women+with+polycystic+ovaries?+A+case-control+study+of+194+treatment+cycles.">In vitro maturation or in vitro fertilization for women with polycystic ovaries? A case-control study of 194 treatment cycles.</a> Fertility and Sterility. 98 (2), 355-360 (2012).</li><li>Ho, V. N. A., Braam, S. C., Pham, T. D., Mol, B. W., Vuong, L. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effectiveness+and+safety+of+in+vitro+maturation+of+oocytes+versus+in+vitro+fertilization+in+women+with+a+high+antral+follicle+count.">The effectiveness and safety of in vitro maturation of oocytes versus in vitro fertilization in women with a high antral follicle count.</a> Human Reproduction. 34 (6), 1055-1064 (2019).</li><li>Sahin, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Which+should+be+the+preferred+technique+during+laparoscopic+ovarian+cystectomy.">Which should be the preferred technique during laparoscopic ovarian cystectomy.</a> Reproductive Sciences. 24 (3), 393-399 (2017).</li><li>Xiao, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+hemostatic+methods+on+ovarian+reserve+and+fertility+in+laparoscopic+ovarian+cystectomy.">Impact of hemostatic methods on ovarian reserve and fertility in laparoscopic ovarian cystectomy.</a> Experimental and Therapeutic. 17 (4), 2689-2693 (2019).</li><li>Song, X. -. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhancing+the+scope+of+in+vitro+maturation+for+fertility+preservation:+transvaginal+retrieval+of+immature+oocytes+during+endoscopic+gynaecological+procedures.">Enhancing the scope of in vitro maturation for fertility preservation: transvaginal retrieval of immature oocytes during endoscopic gynaecological procedures.</a> Human Reproduction. 35 (4), 837-846 (2020).</li><li>Magnusson, &. #. 1. 9. 7. ;., K&#228;llen, K., Thurin-Kjellberg, A., Bergh, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+number+of+oocytes+retrieved+during+IVF:+a+balance+between+efficacy+and+safety.">The number of oocytes retrieved during IVF: a balance between efficacy and safety.</a> Human Reproduction. 33 (1), 58-64 (2018).</li><li>Larose, H., et al., Wellik, D. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gametogenesis:+A+journey+from+inception+to+conception.">Gametogenesis: A journey from inception to conception.</a> Current Topics in Developmental Biology. 132, 257-310 (2019).</li><li>Broer, S. L., Broekmans, F. J. M., Laven, J. S. E., Fauser, B. C. J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-M&#252;llerian+hormone:+ovarian+reserve+testing+and+its+potential+clinical+implications.">Anti-M&#252;llerian hormone: ovarian reserve testing and its potential clinical implications.</a> Human Reproduction Update. 20 (5), 688-701 (2014).</li><li>Arce, J. C., La Marca, A., Mirner Klein, B., Nyboe Andersen, A., Fleming, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antim&#252;llerian+hormone+in+gonadotropin+releasing-hormone+antagonist+cycles:+prediction+of+ovarian+response+and+cumulative+treatment+outcome+in+good-prognosis+patients.">Antim&#252;llerian hormone in gonadotropin releasing-hormone antagonist cycles: prediction of ovarian response and cumulative treatment outcome in good-prognosis patients.</a> Fertility and Sterility. 99 (6), 1644-1653 (2013).</li><li>Katayama, K. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-guided+transvaginal+needle+aspiration+of+follicles+for+in+vitro+fertilization.">Ultrasound-guided transvaginal needle aspiration of follicles for in vitro fertilization.</a> Obstetrics and Gynecology. 72 (2), 271-274 (1988).</li><li>Dellenbach, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transvaginal+sonographically+controlled+follicle+puncture+for+oocyte+retrieval.">Transvaginal sonographically controlled follicle puncture for oocyte retrieval.</a> Fertility and Sterility. 44 (5), 656-662 (1985).</li><li>Hirata, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Concomitant+ovarian+drilling+and+oocyte+retrieval+by+laparoendoscopic+single-site+surgery+led+to+live+birth+using+in+vitro+maturation+of+oocyte+and+transfer+of+frozen-thawed+blastocyst+in+woman+with+polycystic+ovary+syndrome.">Concomitant ovarian drilling and oocyte retrieval by laparoendoscopic single-site surgery led to live birth using in vitro maturation of oocyte and transfer of frozen-thawed blastocyst in woman with polycystic ovary syndrome.</a> Journal of Obstetrics and Gynaecology Research. 40 (5), 1431-1435 (2014).</li><li>Deutinger, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Follicular+aspiration+for+in+vitro+fertilization:+sonographically+guided+transvaginal+versus+laparoscopic+approach.">Follicular aspiration for in vitro fertilization: sonographically guided transvaginal versus laparoscopic approach.</a> European Journal of Obstetrics, Gynecology, and Reproductive Biology. 26 (2), 127-133 (1987).</li><li>Tanbo, T., Henriksen, T., Magnus, O., Abyholm, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Oocyte+retrieval+in+an+IVF+program.+A+comparison+of+laparoscopic+and+transvaginal+ultrasound-guided+follicular+puncture.">Oocyte retrieval in an IVF program. A comparison of laparoscopic and transvaginal ultrasound-guided follicular puncture.</a> Acta Obstetricia et Gynecologica Scandinavica. 67 (3), 243-246 (1988).</li><li>Nagy, Z. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pregnancy+and+birth+after+intracytoplasmic+sperm+injection+of+in+vitro+matured+germinal-vesicle+stage+oocytes:+case+report.">Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report.</a> Fertility and Sterility. 65 (5), 1047-1050 (1996).</li><li>S&#246;derstr&#246;m-Anttila, V., M&#228;kinen, S., Tuuri, T., Suikkari, A. -. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Favourable+pregnancy+results+with+insemination+of+in+vitro+matured+oocytes+from+unstimulated+patients.">Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients.</a> Human Reproduction. 20 (6), 1534-1540 (2005).</li><li>Hwang, J. L., Lin, Y. H., Tsai, Y. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+maturation+and+fertilization+of+immature+oocytes:+a+comparative+study+of+fertilization+techniques.">In vitro maturation and fertilization of immature oocytes: a comparative study of fertilization techniques.</a> Journal of Assisted Reproduction and Genetics. 17 (1), 39-43 (2000).</li><li>Walls, M., Junk, S., Ryan, J. 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本文中描述的OP-IVM方法扩展到传统的IVM应用，并将卵母细胞取回后的IVM与常规妇科手术相结合。在妇科手术中会丢失的卵母细胞现在可用于IVF-ET或FP，而无需额外的手术风险。OP-IVM首先用于在PCOS患者卵巢钻孔之前取回卵母细胞。其应用很快扩展到需要良性妇科手术的不孕患者和需要放化疗的癌症或血液疾病患者。由于存在潜在的转移风险，OP-IVM不适合或推荐用于恶性肿瘤患者。基于OP-IVM，该机构?...

An erratum was issued for:&#160;<a href="https://www.jove.com/t/61647">OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery</a>. The Protocol and Representative Results sections were&#160;updated.
Step 3.2 of the Protocol was updated from:
Add 0.5 mL of IVM&#160;medium supplemented with 0.075 IU/mL of follicle-stimulating hormone (FSH) and 0.075 IU/mL of luteinizing hormone (LH) to each well of a 4-well plate. Cover the medium with oil.
to:
Add 0.5 mL of IVM&#160;medium supplemented with 0.75 IU/mL of follicle-stimulating hormone (FSH) and 0.75 IU/mL of luteinizing hormone (LH) to each well of a 4-well plate. Cover the medium with oil.
The legend for Figure 2 was updated from:
Figure 2:&#160;Morphological characteristics of representative images of oocytes. (A) COCs, (B) MII, (C) MI, (D) GV stage. Abbreviations: COCs = cumulus-oocyte complexes; GV = germinal vesicle; MI = metaphase I; MII = metaphase II.
to:
Figure 2:&#160;Morphological characteristics of representative images of oocytes. (A) COCs, (B) GV, (C) MI, (D) MII&#160;stage. Abbreviations: COCs = cumulus-oocyte complexes; GV = germinal vesicle; MI = metaphase I; MII = metaphase II.

An erratum was issued for:&#160;<a href="https://www.jove.com/t/61647">OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery</a>. The Protocol and Representative Results sections were&#160;updated.

Erratum: OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

IVM是一种ART，其中人未成熟的卵母细胞在体外培养至IVF-ET或FP成熟。在IVM中，不使用排卵诱导药物，从而减轻疼痛，经济负担和并发症，如卵巢过度刺激综合征（OHSS）1，2。此外，IVM特别适用于癌症患者的FP和不孕症患者的不孕症治疗，他们可能无法或没有时间接受排卵诱导治疗3。因此，虽然取回的卵母细胞数量、临床妊娠率（CPR）和活产率（LBR）均低于IVF4、5，但IVM有其独特的优势。
患有子宫内膜病变、输卵管积水或卵巢囊肿的不孕患者通常在 ART 治疗前进行妇科手术，其卵母细胞通常不成熟。OP-IVM使用引导经阴道超声来检索未成熟的卵母细胞并在体外生长，直到IVF-ET或FP成熟。OP-IVM在卵母细胞取回和妇科手术后结合IVM，从而减少受控卵巢过度刺激周期中常见的并发症，节省时间和金钱。对于有生育能力的患者，OP-IVM可以作为接受常规妇科手术的"生育保险"。
此外，妇科手术造成的损害，如电烙术6，7和卵巢肿瘤切除术，可以通过妇科手术前的卵母细胞取回来减少。因此，与常规妇科手术相比，OP-IVM可以减少不孕症治疗期间的手术次数，防止卵巢手术期间功能性卵母细胞的丧失。
先前的一项研究表明，额外的卵母细胞取回程序既不会增加手术并发症和不良妊娠结局，也不会延长住院时间8。一些患者通过OP-IVM8进行了活产，这表明这种方法的可行性。本文描述了可能受益于OP-IVM的患者的特征以及OP-IVM的程序和关键点，并讨论了人类卵母细胞成熟的评估。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>19 G single-lumen aspiration needles</td>
			<td>Cook, Australia</td>
			<td>K-OPS-7035-REH-ET</td>
			<td></td>
		</tr>
		<tr>
			<td>4-well plate</td>
			<td>Corning</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>70 &#956;m nylon cell strainer</td>
			<td>Falcon, USA</td>
			<td>352350</td>
			<td></td>
		</tr>
		<tr>
			<td>CO2 Incubator</td>
			<td>Thermo</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Culture oil</td>
			<td>Vitrolife, Sweden</td>
			<td>10029,OVOIL&#160;</td>
			<td>Step 3.2.</td>
		</tr>
		<tr>
			<td>FSH &#38; LH</td>
			<td>Ferring Reproductive Health, Germany</td>
			<td>MENOPUR&#174;</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass Pasteur pipette</td>
			<td>Hilgenberg GmbH, Germany</td>
			<td>3154102-26</td>
			<td></td>
		</tr>
		<tr>
			<td>G-MOPS medium</td>
			<td></td>
			<td></td>
			<td>pH-stable handling medium for washing the needle before puncturing</td>
		</tr>
		<tr>
			<td>IVM medium</td>
			<td>Origio, Denmark</td>
			<td>ART-1600-B</td>
			<td></td>
		</tr>
		<tr>
			<td>Laminar Flow Clean Benches</td>
			<td>ESCO</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Petri dish</td>
			<td>Thermo Fisher Scientific, Denmark</td>
			<td>263991</td>
			<td></td>
		</tr>
		<tr>
			<td>pH stable handing media designed to support the handling and manipulation of oocytes and embryos outside the incubator</td>
			<td>Vitrolife, Sweden</td>
			<td>10130, G-MOPS PLUS</td>
			<td>Step 7.1.</td>
		</tr>
		<tr>
			<td>Rinse solution</td>
			<td>Cook, Australia</td>
			<td>K-SIFB-100</td>
			<td></td>
		</tr>
		<tr>
			<td>Stereoscope</td>
			<td>Nikon</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

op-ivm: combining in vitro maturation after oocyte retrieval with gynecological surgery

妇科手术前体外成熟（IVM）的使用（OP-IVM）是传统IVM的延伸，它将卵母细胞取回后的IVM与常规妇科手术相结合。OP-IVM适用于接受良性妇科手术的患者，他们需要保留生育能力（FP）或不孕症治疗，如体外受精和胚胎移植（IVF-ET）。在手术室中，接受良性妇科手术的患者首先被麻醉并接受超声引导的未成熟卵泡抽吸（IMFA）治疗。随着随后的妇科手术的进行，检查积云 - 卵母细胞复合物（COCs），并将未成熟的COCs转移到IVM培养基中并在IVF实验室中培养28-32小时。评估后，将选择MII阶段的成熟卵母细胞并将其冷冻保存在液氮中用于FP或通过卵胞浆内单精子注射（ICSI）受精用于IVF-ET。通过将IVM与妇科手术相结合，可以保存本来会丢弃的未成熟卵母细胞并将其用于辅助生殖技术（ART）。本文介绍了OP-IVM的程序，重要性和关键方面。

截至2019年12月，OP-IVM用于274名患者的生育能力保存。2014年至2016年间158名患者的胚胎学和生殖结局发表在之前的论文8中。以下示例讨论了2016年接受OP-IVM的PCOS患者所遵循的程序。患者为28岁，被诊断为原发性不孕、左附件囊肿和PCOS。她于2016年9月28日接受了腹腔镜膀胱切除术和OP-IVM手术。获得27个未成熟的COC。培养28小时后，选取7个MII阶段的卵母细...

Watch this Scientific Journal Video about OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery at JoVE.com

OP-IVM: Combining In vitro Maturation after Oocyte Retrieval with Gynecological Surgery

妇科手术前体外成熟（IVM）（OP-IVM）将卵母细胞取回后的IVM与常规妇科手术相结合，并作为常规IVM应用的延伸，用于保留生育能力。

OP-IVM：将卵母细胞取回后的体外成熟与妇科手术相结合

The use of in vitro maturation (IVM) before gynecological operation (OP-IVM) is an extension of conventional IVM that combines IVM following oocyte ...

OP-IVM技术是传统IVM方案的扩展，将卵母细胞取回后的IVM与常规妇科手术相结合。适用于在ART治疗或生育力保存前需要接受妇科手术的不孕或亚孕妇女，需要腹腔镜卵巢钻孔手术的克罗米芬抵抗多囊卵巢综合征患者，需要宫腔镜子宫肌瘤切除术，息肉切除术，经胸切除术和腹腔镜管手术等良性妇科手术的不孕患者， 和ART治疗前的卵巢切除术，以及正在接受放化疗或放疗的癌症和血液系统疾病患者，他们可以进行这种手术以保持生育能力。将超声探头放在阴道内扫描并记录两个卵巢中的卵泡数量。找到离卵巢最近的位置作为穿刺部位，避开肠道、膀胱和大血管。用pH稳定的处理介质清洗19号单腔抽吸针，然后在超声波的指导下将针头注射到卵巢中。在80至90毫米汞柱的压力下用针头吸出卵泡液，稍微旋转针头以吸出尽可能多的卵泡液。使用肝素降低抽吸过程中的滤泡液粘度。调整探针的位置，使其始终尽可能靠近卵巢，并用适当的力按压阴道穹窿，以减少损伤和出血。确保在 25 至 30 分钟内完成卵泡液抽吸。在一个卵巢中完成后拔出针头，用处理介质清洗针头，然后使用相同的方法刺穿另一侧。在37摄氏度的同温平板中工作，准备处理培养基，培养皿和细胞过滤器。用预热的pH稳定处理介质冲洗培养管和过滤器。用70微米尼龙细胞过滤器过滤吸入的卵泡液，用预热的pH稳定处理介质反复冲洗培养管和过滤器。确保将所有未成熟的COC完全转移到培养皿中。将血液转移到干净的处理培养基中。然后，将细胞过滤器转移到新培养皿中并清洗。吸出处理培养基，并将处理培养基与COC，颗粒细胞和组织转移到新培养皿中。以40倍放大倍率检查立体镜下的COC。将未成熟物快速转移到预热的IVM卵母细胞培养基中。接下来，检查血凝块和细胞过滤器中的COC。将COCs和处理培养基转移到预热的IVM卵母细胞培养基中。将未成熟的COCs在37摄氏度的含5%二氧化碳和5%氧气的加湿空气中培养28至32小时。截至2019年12月，OP-IVM已被用于274名患者的生育能力保存。一个例子是一名28岁的患者，他被诊断出患有原发性不孕症，左附件囊肿和PCOS。她于2016年9月接受了腹腔镜膀胱切除术和OP-IVM手术，并获得了27个未成熟的COC。培养28小时后，选取7个MII期卵母细胞，由ICSI施肥。五天后，两个囊胚被冷冻。其中一个在2017年2月解冻并转移到子宫中。病人在11月生下了一个健康的女孩。OP-IVM首先应用于正在接受卵巢钻孔手术的PCOS不孕妇女的卵母细胞取卵，现在其应用可以扩展到需要接受良性妇科手术的不育妇女，甚至是癌症妇女的生育能力保存。它表明，通过将其与其他传统妇科手术相结合，可以扩大IVM的应用范围，以保持女性的生育能力。

Research

JoVE Journal

Medicine

Step By Step: Microsurgical training method combining two nonliving animal models

一步一步：显微外科培训方法相结合的两个无生命的动物模型

The learning of microsurgical techniques and the maintenance of microsurgical skills have been traditionally based on the use of living animals, mainly ...

科研

医学

A Case Series of Successful Abdominal Closure Utilizing a Novel Technique Combining a Mechanical Closure System with a Biologic Xenograft that Accelerates Wound Healing

利用一种新技术,将机械闭合系统与可加速伤口愈合的生物异种移植相结合的成功腹部闭合案例系列

In the acute setting, once intra-abdominal injuries have been addressed, the next great hurdle is restoring a functional and intact abdominal compartment. ...

Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis

视频栅格立体成像和跑步机步态分析对腰椎融合手术后患者体型和步态剖面的评价

This protocol provides guidance on how to perform high resolution video rasterstereography and treadmill gait analysis on patients after lumbar fusion ...

Structured Motor Rehabilitation After Selective Nerve Transfers

选择性神经转移后的结构化运动康复

After severe nerve injuries, selective nerve transfers provide an opportunity to restore motor and sensory function. Functional recovery depends both on ...

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

大鼠胡萝卜动脉压力控制段气球损伤与腹腔ventit 治疗应用

Cardiovascular disease remains the leading cause of death and disability worldwide, in part due to atherosclerosis. Atherosclerotic plaque narrows the ...

Combining Augmented Reality and 3D Printing to Display Patient Models on a Smartphone

结合增强现实和 3D 打印，在智能手机上显示患者模型

Augmented reality (AR) has great potential in education, training, and surgical guidance in the medical field. Its combination with three-dimensional (3D) ...

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

使用GCaMP3报告器测量心脏特异性钙通量，对心脏重编程进行体外评估

Cardiac reprogramming has become a potentially promising therapy to repair a damaged heart. By introducing multiple transcription factors, including ...

Combining 3D-Printing and Electrospinning to Manufacture Biomimetic Heart Valve Leaflets

结合3D打印和静电纺丝来制造仿生心脏瓣膜传单

Electrospinning has become a widely used technique in cardiovascular tissue engineering as it offers the possibility to create (micro-)fibrous scaffolds ...

Fertility Sparing Procedure using Carbon Dioxide Fiber Laser Vaporization of Ovarian Endometrioma

使用二氧化碳光纤激光汽化卵巢子宫内膜异位瘤的生育保留程序

The surgical management of endometrioma is still a matter of debate. Cystectomy, which is recognized as the standard technique, seems to be associated ...

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

In Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKHS) patients who are scheduled for laparoscopic vaginoplasty and have a desire for biological motherhood, ...