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DOI :
10.3791/61648-v
September 15th, 2020
Chapters
0:05
Introduction
0:51
Equipment Preparation
3:11
3’-End Biotinylated mRNA Immobilization
5:20
Translation Mix Assembly and Delivery
7:24
Results: Analyses for Saccharomyces cerevisiae Translation of a Firefly Luciferase-encoding mRNA
9:12
Conclusion
跟踪单个翻译事件允许对依赖上限的翻译机制进行高分辨率动能研究。在这里,我们演示了基于荧光标记抗体和表皮标记的新生肽之间的成像相互作用的 体外 单分子检测。该方法使启动和肽伸长动力学的单分子特征 在主动体外 帽依赖翻译。
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