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DOI :
10.3791/61675-v
•
7:47 min
September 26th, 2020
Chapters
0:00
Introduction
0:42
Surgical Procedure
1:18
Preparing the Surgical Field
1:31
Left Lateral Thoracotomy
2:00
Left Anterior Descending (LAD) Permanent Coronary Artery Ligation
2:58
3D Bioprinting and Culturing Patches (7-14 days before transplantation)
4:19
Transplantation of the Bioprinted Patch onto the Epicardium
5:39
Mouse Recovery
6:29
Representative Results
6:45
Conclusion
该协议旨在移植一个3D生物打印补丁到梗死小鼠的史诗建模心力衰竭。它包括有关麻醉、手术胸腔开口、左前降(LAD)冠状动脉的永久结扎以及将生物打印的贴片应用到心脏梗塞区域的详细信息。
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