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JoVE Journal

Bioengineering

Author Produced

This content is Open Access.

Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction

6.4K Views

07:47 min

September 26th, 2020

September 26th, 2020

6,371 Views

0:00

Introduction

0:42

Surgical Procedure

1:18

Preparing the Surgical Field

1:31

Left Lateral Thoracotomy

2:00

Left Anterior Descending (LAD) Permanent Coronary Artery Ligation

2:58

3D Bioprinting and Culturing Patches (7-14 days before transplantation)

4:19

Transplantation of the Bioprinted Patch onto the Epicardium

5:39

Mouse Recovery

6:29

Representative Results

6:45

Conclusion

Transcript

This protocol aims to transplant a 3D bioprinted patch onto the epicardium of infarcted mice modeling heart failure. It includes details regarding anesthesia, the surgical chest opening, permanent ligation of the left anterior descending (LAD) coronary artery and application of a bioprinted patch onto the infarcted area of the heart.

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