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DOI :
10.3791/61675-v
•
7:47 min
September 26th, 2020
Chapters
0:00
Introduction
0:42
Surgical Procedure
1:18
Preparing the Surgical Field
1:31
Left Lateral Thoracotomy
2:00
Left Anterior Descending (LAD) Permanent Coronary Artery Ligation
2:58
3D Bioprinting and Culturing Patches (7-14 days before transplantation)
4:19
Transplantation of the Bioprinted Patch onto the Epicardium
5:39
Mouse Recovery
6:29
Representative Results
6:45
Conclusion
このプロトコルは、心不全をモデル化する梗塞マウスの心外膜に3Dバイオプリントパッチを移植することを目的としている。麻酔、手術胸部開口部、左前下降(LAD)冠動脈の永久結紮、心臓の梗塞領域へのバイオプリントパッチの適用に関する詳細が含まれています。
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