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DOI :
10.3791/61680-v
September 20th, 2020
Chapters
0:04
Introduction
0:34
RNA Fluorescence In Situ Hybridization (FISH) Fixation
1:14
RNA FISH Hybridization
2:16
Imaging Preparation
3:08
High Resolution Microscopy and Image Processing
4:05
Image Analysis
4:45
Results: Representative Image Segmentation and E2-Induced GREB1 Quantification
5:56
Conclusion
单分子RNA荧光就地杂交(smFISH)是一种在单细胞水平上准确量化特定RNA水平和定位的方法。在这里,我们报告我们已验证的实验室协议,用于湿板凳处理、成像和图像分析,用于特定RNA的单细胞量化。
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