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DOI :
10.3791/61680-v
September 20th, 2020
Chapters
0:04
Introduction
0:34
RNA Fluorescence In Situ Hybridization (FISH) Fixation
1:14
RNA FISH Hybridization
2:16
Imaging Preparation
3:08
High Resolution Microscopy and Image Processing
4:05
Image Analysis
4:45
Results: Representative Image Segmentation and E2-Induced GREB1 Quantification
5:56
Conclusion
この場合の単一分子RNA蛍光蛍光(smFISH)は、単一細胞レベルで特異的なRNAのレベルおよび局在を正確に定量する方法です。ここでは、特定のRNAの単一細胞定量化のためのウェットベンチ処理、イメージングおよび画像解析のための検証済みのラボプロトコルを報告します。
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