Name: operating transverse aortic constriction with absorbable suture to obtain transient myocardial hypertrophy
Uploaded: 2020-09-09T14:45:00
Description: Watch this Scientific Journal Video about Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy at JoVE.com

这项工作得到了中国国家自然科学基金委员会（81770271：给Y，廖），中国国家自然科学基金联合基金（U1908205：给Y，廖）和广州市科技规划项目（201804020083：廖博士）的资助。

所有程序都是按照美国国家卫生研究院出版的《 实验室动物的护理和使用 指南》进行的（NIH出版物第85-23号，1996年修订）。C57BL/6J雄性小鼠（8~10周，20~25克）由南方医科大学动物中心提供。
1. 术前准备
<ol><li>用针架捏掉 25 G 针的尖端，用像支架这样的硬物将其钝化。</li>
	<li>通过针头传递一个5+0可吸收的缝合线，然后弯曲到90°与持有人13。 注：?...

<ol>
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T., Flo, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regression+of+left+ventricular+hypertrophy+during+10+years+after+valve+replacement+for+aortic+stenosis+is+related+to+the+preoperative+risk+profile.">Regression of left ventricular hypertrophy during 10 years after valve replacement for aortic stenosis is related to the preoperative risk profile.</a> European Heart Journal. 24 (15), 1437-1446 (2003).</li><li>Rockman, H. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segregation+of+atrial-specific+and+inducible+expression+of+an+atrial+natriuretic+factor+transgene+in+an+in+vivo+murine+model+of+cardiac+hypertrophy.">Segregation of atrial-specific and inducible expression of an atrial natriuretic factor transgene in an in vivo murine model of cardiac hypertrophy.</a> Proceedings of the National Academy of Sciiences of the United States of America. 88 (18), 8277-8281 (1991).</li><li>Wei, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+hypertrophic+preconditioning+attenuates+cardiomyocyte+hypertrophy+and+slows+progression+to+heart+failure+through+upregulation+of+S100A8/A9.">Myocardial hypertrophic preconditioning attenuates cardiomyocyte hypertrophy and slows progression to heart failure through upregulation of S100A8/A9.</a> Circulation. 131 (17), 1506-1517 (2015).</li><li>Huang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+biomicroscopy+validation+of+a+murine+model+of+cardiac+hypertrophic+preconditioning:+comparison+with+a+hemodynamic+assessment.">Ultrasound biomicroscopy validation of a murine model of cardiac hypertrophic preconditioning: comparison with a hemodynamic assessment.</a> American Journal of Physiology. 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在心脏非缺血预科中，仍然存在着一个大大不足的区域。根据我们以前的研究，我们改用可吸收的缝合线来改善心肌肥大预后模式。
在先前的报告中，许多调查人员使用丝绸缝合来收缩主动脉拱门8号、14号、15号。丝绸缝合很容易获得，经常用于手术伤口缝合，组织结块和组织固定。在此协议中，我们用?...

缺血性先导是一种现象，诱发短暂的非致命性缺血发作和对心脏的再灌注，并有能力显著减少心肌损伤1。鉴于缺血性先决条件的明显临床意义，如限制心肌梗塞大小2和抑制心室心肌梗塞后心肌复明3，已经有很多研究来解剖机制潜在的心防作用诱导的先决条件4，5。相比之下，其他非缺血的先决条件却很少受到关注。心脏肥大可能钝化在主动脉狭窄患者进行主动脉瓣更换6。在存在病理心肌萎缩状态的地方，很少报告先决条件原理。
1991年，罗克曼等人首次建立了通过横向主动脉收缩（TAC）7导致左心室肥大的小鼠模型。通过在小鼠中两次操作TAC，我们先前已经证明心肌肥大预后（MHP）会导致心脏短暂性肥大刺激，从而使心脏在未来8月对持续的肥大压力更具抵抗力。MHP模型的特点已通过超声波生物显微镜和血动力学评估9得到验证。构建模型的要点是执行胸腔切除术三次，TAC 一周，解散一周，二级 TAC 6 周。然而，去组织可能导致出血，这使得新手难以掌握，也难以推广。此外，给小鼠进行管网也是一项技术挑战。不当的受管可能导致气管损伤、肺气管损伤，甚至导致小鼠死亡。因此，在构建MHP模型的同时，改进一些程序是必要和有价值的。
为了降低模型的难度并增加其成功率，我们切换到可吸收的缝合线为第一个TAC，并通过测量压力梯度通过回声心动图10下主动脉收缩监测模型的成功。根据我们的初步实验，在压力梯度过低的小鼠中很难诱发足够的心肌肥大，而压力梯度过高的小鼠会发展成急性心力衰竭甚至死亡。模型的理想压力梯度范围为 40–80 mmHg11。此外，这个实验没有依靠呼吸机，它可以有效地避免呼吸机相关的技术操作和伤害12。

<table>
	<tbody>
		<tr>
			<td>Absorbable suture (5-0)</td>
			<td>Shandong Kang Lida Medical Products Co., Ltd</td>
			<td>5-0</td>
			<td>Ligation</td>
		</tr>
		<tr>
			<td>Animal ultrasound system VEVO2100</td>
			<td>Visual Sonic</td>
			<td>VEVO2100</td>
			<td>Echocardiography</td>
		</tr>
		<tr>
			<td>Cold light illuminator</td>
			<td>Olympus</td>
			<td>ILD-2</td>
			<td>Light</td>
		</tr>
		<tr>
			<td>Heat pad- thermostatic surgical system (ALC-HTP-S1)</td>
			<td>SHANGHAI ALCOTT BIOTECH CO</td>
			<td>ALC-HTP-S1</td>
			<td>Heating</td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>RWD life science</td>
			<td>R510-22</td>
			<td>Inhalant anaesthesia</td>
		</tr>
		<tr>
			<td>Matrx VIP 3000 Isofurane Vaporizer</td>
			<td>Midmark Corporation</td>
			<td>VIP 3000</td>
			<td>Anesthetization</td>
		</tr>
		<tr>
			<td>Medical nylon suture (5-0)</td>
			<td>Ningbo Medical Needle Co.</td>
			<td>5-0</td>
			<td>Close the skin</td>
		</tr>
		<tr>
			<td>Pentobarbital sodium salt</td>
			<td>Merck</td>
			<td>25MG</td>
			<td>Anesthetization</td>
		</tr>
		<tr>
			<td>Precision electronic balance</td>
			<td>Denver Instrument</td>
			<td>TB-114</td>
			<td>Weighing sensor</td>
		</tr>
		<tr>
			<td>Self-made spacer</td>
			<td></td>
			<td></td>
			<td>25-gauge needle</td>
		</tr>
		<tr>
			<td>Silk suture (5-0)</td>
			<td>Yangzhou Yuankang Medical Devices Co., Ltd.</td>
			<td>5-0</td>
			<td>Ligation</td>
		</tr>
		<tr>
			<td>Small animal microsurgery equipment</td>
			<td>Napox</td>
			<td>MA-65</td>
			<td>Surgical instruments</td>
		</tr>
		<tr>
			<td>Transmission Gel</td>
			<td>Guang Gong pai</td>
			<td>250ML</td>
			<td>Echocardiography</td>
		</tr>
		<tr>
			<td>Veet hair removal cream</td>
			<td>Reckitt Benchiser</td>
			<td>RQ/B 33 Type 2</td>
			<td>Remove hair of mice</td>
		</tr>
		<tr>
			<td>Vertical automatic electrothermal pressure steam sterilizer</td>
			<td>Hefei Huatai Medical Equipment Co.</td>
			<td>LX-B50L</td>
			<td>Auto clean the surgical instruments</td>
		</tr>
	</tbody>
</table>

operating transverse aortic constriction with absorbable suture to obtain transient myocardial hypertrophy

基于小鼠两次横向主动脉收缩（TACs），证明心肌肥大预后（MHP）可以减轻心肌细胞肥大，减缓心力衰竭的进展。然而，对于新手来说，MHP模型通常很难建立，因为呼吸机操作的技术障碍，反复打开胸部，以及脱体引起的出血。为了促进这种模式，提高手术成功率和减少出血的发生率，我们改用可吸收的缝合线进行第一次TAC梳理，采用无呼吸机技术。使用2周的可吸收缝合，我们证明这个程序可能会导致严重的心肌萎缩在2周：手术后4周，心肌萎缩几乎完全退回到基线。使用此协议，操作员可以轻松掌握 MHP 模型，降低操作死亡率。

在这项研究中，我们随机将45只小鼠分成三组，分别是假象组、丝缝组和可吸收缝合组（TAC之后D0（基线）、D14和D28上每组的数量分别为15、10和5）。手术后的D7、D14、D21和D28，收缩的峰值速度由心电图确定。我们发现，在TAC之后的第二周，收缩处的血流速度仍然大于3，000毫米/秒，即使使用可吸收的缝合线收缩主动脉拱门（图1A）。此外，可吸收缝合组收缩时的压力梯度在2周...

Watch this Scientific Journal Video about Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy at JoVE.com

Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy

该协议提出了一个改进的方法，以获得可吸收的缝合的瞬时心肌肥大，模拟左心室肥大减少后，消除压力过载。它可能对心肌肥大先天性研究很有价值。

操作横向主动脉收缩与可吸收的缝合获得瞬时心肌萎缩

Based on twice transverse aortic constrictions (TACs) in mice, it is proved that myocardial hypertrophic preconditioning (MHP) could attenuate ...

Research

JoVE Journal

Medicine

A Research Method For Detecting Transient Myocardial Ischemia In Patients With Suspected Acute Coronary Syndrome Using Continuous ST-segment Analysis

研究方法检测疑似急性冠脉综合征患者短暂心肌缺血的连续ST段分析

Each year, an estimated 785,000 Americans will have a new coronary attack, or acute coronary syndrome (ACS). The pathophysiology of ACS involves rupture ...

科研

医学

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest

心肌顿抑相关的小鼠离体心脏模型心脏停搏

The following protocol is of use to evaluate impaired cardiac function or myocardial stunning following moderate ischemic insults. The technique is useful ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Minimally Invasive Transverse Aortic Constriction in Mice

微创横向主动脉缩窄小鼠

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots

全根主动脉瓣置换主动脉瓣狭窄主动脉异种移植患者小主动脉根

In patients with small aortic roots who need an aortic valve replacement with biological valve substitutes, the implantation of the stented pericardial ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

小鼠横主动脉狭窄诱导左心室肥厚的技术研究

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

诱导小鼠横向主动脉狭窄的闭合胸模型

Research on cardiac hypertrophy and heart failure is frequently based on pressure overload mouse models induced by TAC. The standard procedure is to ...

Laparoscopic Repair of Para-Esophageal Hernia Using Absorbable Biosynthetic Mesh

使用可吸收的生物合成网格对副食管气喘进行拉帕罗镜修复

Para-esophageal hernia repairs are challenging procedures and there is no consensus on the optimal approach to repair. Mesh reinforcement has been ...

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

使用改性非缝合铐技术的宫颈主动脉移植模型

With the introduction of powerful immunosuppressive protocols, distinct advances are possible in the prevention and therapy of acute rejection episodes. ...