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Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

DOI :

10.3791/61704-v

August 28th, 2020

August 28th, 2020

7,997 Views

1Department of Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University

This protocol describes the preparation of horizontal hippocampal-entorhinal cortex (HEC) slices from mice exhibiting spontaneous sharp-wave ripple activity. Slices are incubated in a simplified interface holding chamber and recordings are performed under submerged conditions with fast-flowing artificial cerebrospinal fluid to promote tissue oxygenation and the spontaneous emergence of network-level activity.

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Acute Mouse Brain Slicing

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