JoVE Logo
Faculty Resource Center

Sign In

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

DOI :

10.3791/51958-v

September 28th, 2014

September 28th, 2014

14,335 Views

1Department of Physiology and Centre for Brain Research, University of Auckland, 2Department of Molecular and Cellular Physiology, Stanford University

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

Tags

Paired Whole Cell Recordings

-- Views

Related Videos

article

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

article

Organotypic Hippocampal Slice Cultures

article

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

article

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

article

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

article

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

article

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

article

Whole-cell Patch-clamp Recordings in Brain Slices

article

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

article

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved