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DOI :
10.3791/61739-v
•
6:12 min
January 27th, 2021
Chapters
0:05
Introduction
0:35
PL, IPL, and OPL Samplings
2:44
Sample Treatment for Protein Solubilization and SDS-PAGE Analyses
4:37
Results: SDS-PAGE Analysis of Separated OPL and IPL from Freshly Laid Unfertilized Egg
5:27
Conclusion
この記事では、鶏卵の外側と内側の膜下層を分離しながら、構造変化を最小限に抑え、プロテオミクス分析のために各サブ層のタンパク質可溶化を最適化する簡単な方法を報告します。
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