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DOI :
10.3791/61751-v
December 5th, 2020
Chapters
0:05
Introduction
0:55
Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
2:23
CSC Passage
3:24
HUEhT-1 Endothelial Cell Culture
4:14
NICO-1 Coculture Plate Preparation
5:16
NICO-1 System HUEhT-1 and CSC Seeding
6:14
Results: Representative CSC-Induced Neovascularization and Vascular Formation
7:19
Conclusion
卵巢癌干细胞 (OCSC) 负责癌症的发生、复发、治疗耐药性和转移。OCSC血管生态位被认为可促进OCSC的自我更新,导致化学耐药性。该协议为体外建立可重复的OCSC血管生态位模型提供了基础。
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