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DOI :
10.3791/61751-v
December 5th, 2020
Chapters
0:05
Introduction
0:55
Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
2:23
CSC Passage
3:24
HUEhT-1 Endothelial Cell Culture
4:14
NICO-1 Coculture Plate Preparation
5:16
NICO-1 System HUEhT-1 and CSC Seeding
6:14
Results: Representative CSC-Induced Neovascularization and Vascular Formation
7:19
Conclusion
卵巣がん幹細胞(OCSC)は、がんの開始、再発、治療抵抗性、および転移の原因です。OCSC血管ニッチは、OCSCの自己複製を促進し、化学療法抵抗性につながると考えられています。このプロトコルは、in vitroで再現可能なOCSC血管ニッチモデルを確立するための基礎を提供します。
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