Name: intramyocardial transplantation of msc-loading injectable hydrogels after myocardial infarction in a murine model
Uploaded: 2020-09-20T15:00:00
Description: Watch this Scientific Journal Video about Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model at JoVE.com

Questa ricerca è supportata dal Basic Science Research Program attraverso la National Research Foundation of Korea (NRF) finanziata dal Ministero dell'Istruzione (NRF-2018R1D1A1A02049346)

Tutte le procedure di ricerca sugli animali sono state fornite in conformità con il Laboratory Animals Welfare Act, la Guida per la cura e l'uso degli animali da laboratorio e le linee guida e le politiche per gli esperimenti sui roditori fornite dall'Institutional Animal Care and Use Committee (IACUC) nella School of Medicine dell'Università Cattolica della Corea.
1. Preparazione di CSC e idrogel di gelatina iniettabili
<ol><li>CCI della cultura in un piatto da coltura da 100 mm a 37 °C ...

<ol>
	<li>Jhund, P. S., McMurray, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+failure+after+acute+myocardial+infarction:+a+lost+battle+in+the+war+on+heart+failure.">Heart failure after acute myocardial infarction: a lost battle in the war on heart failure.</a> Circulation. 118 (20), 2019-2021 (2008).</li><li>Cahill, T. J., Kharbanda, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heart+failure+after+myocardial+infarction+in+the+era+of+primary+percutaneous+coronary+intervention:+Mechanisms,+incidence+and+identification+of+patients+at+risk.">Heart failure after myocardial infarction in the era of primary percutaneous coronary intervention: Mechanisms, incidence and identification of patients at risk.</a> World Journal of Cardiology. 9 (5), 407-415 (2017).</li><li>Cambria, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translational+cardiac+stem+cell+therapy:+advancing+from+first-generation+to+next-generation+cell+types.">Translational cardiac stem cell therapy: advancing from first-generation to next-generation cell types.</a> npj Regenerative Medicine. 2, 17 (2017).</li><li>Lemcke, H., Voronina, N., Steinhoff, G., David, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+Progress+in+Stem+Cell+Modification+for+Cardiac+Regeneration.">Recent Progress in Stem Cell Modification for Cardiac Regeneration.</a> Stem Cells International. 2018, 1909346 (2018).</li><li>Alagarsamy, K. N., Yan, W., Srivastava, A., Desiderio, V., Dhingra, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+injectable+hydrogels+for+cardiac+stem+cell+therapy+and+tissue+engineering.">Application of injectable hydrogels for cardiac stem cell therapy and tissue engineering.</a> Reviews in Cardiovascular Medicine. 20 (4), 221-230 (2019).</li><li>Gaetani, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epicardial+application+of+cardiac+progenitor+cells+in+a+3D-printed+gelatin/hyaluronic+acid+patch+preserves+cardiac+function+after+myocardial+infarction.">Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction.</a> Biomaterials. 61, 339-348 (2015).</li><li>Gao, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+Tissue+Engineering+With+Cells+Derived+From+Human-Induced+Pluripotent+Stem+Cells+and+a+Native-Like,+High-Resolution,+3-Dimensionally+Printed+Scaffold.">Myocardial Tissue Engineering With Cells Derived From Human-Induced Pluripotent Stem Cells and a Native-Like, High-Resolution, 3-Dimensionally Printed Scaffold.</a> Circualtion Research. 120 (8), 1318-1325 (2017).</li><li>Hasan, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injectable+Hydrogels+for+Cardiac+Tissue+Repair+after+Myocardial+Infarction.">Injectable Hydrogels for Cardiac Tissue Repair after Myocardial Infarction.</a> Advanced Science. 2 (11), 1500122 (2015).</li><li>Wu, R., Hu, X., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Concise+Review:+Optimized+Strategies+for+Stem+Cell-Based+Therapy+in+Myocardial+Repair:+Clinical+Translatability+and+Potential+Limitation.">Concise Review: Optimized Strategies for Stem Cell-Based Therapy in Myocardial Repair: Clinical Translatability and Potential Limitation.</a> Stem Cells. 36 (4), 482-500 (2018).</li><li>Lee, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+situ+forming+gelatin-based+tissue+adhesives+and+their+phenolic+content-driven+properties.">In situ forming gelatin-based tissue adhesives and their phenolic content-driven properties.</a> Journal of Materials Chemistry B. 1 (18), 2407-2414 (2013).</li><li>Lee, Y., Bae, J. W., Lee, J. W., Suh, W., Park, K. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enzyme-catalyzed+in+situ+forming+gelatin+hydrogels+as+bioactive+wound+dressings:+effects+of+fibroblast+delivery+on+wound+healing+efficacy.">Enzyme-catalyzed in situ forming gelatin hydrogels as bioactive wound dressings: effects of fibroblast delivery on wound healing efficacy.</a> Journal of Materials Chemistry B. 2 (44), 7712-7718 (2014).</li><li>Lee, S. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+situ+Crosslinkable+Gelatin+Hydrogels+for+Vasculogenic+Induction+and+Delivery+of+Mesenchymal+Stem+Cells.">In situ Crosslinkable Gelatin Hydrogels for Vasculogenic Induction and Delivery of Mesenchymal Stem Cells.</a> Advanced Functional Materials. 24 (43), 6771-6781 (2014).</li><li>Jung, B. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+hydrogel+matrix+prolongs+persistence+and+promotes+specific+localization+of+an+oncolytic+adenovirus+in+a+tumor+by+restricting+nonspecific+shedding+and+an+antiviral+immune+response.">A hydrogel matrix prolongs persistence and promotes specific localization of an oncolytic adenovirus in a tumor by restricting nonspecific shedding and an antiviral immune response.</a> Biomaterials. 147, 26-38 (2017).</li><li>Kim, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tonsil-derived+mesenchymal+stem+cell-embedded+in+situ+crosslinkable+gelatin+hydrogel+therapy+recovers+postmenopausal+osteoporosis+through+bone+regeneration.">Tonsil-derived mesenchymal stem cell-embedded in situ crosslinkable gelatin hydrogel therapy recovers postmenopausal osteoporosis through bone regeneration.</a> PLoS One. 13 (7), 0200111 (2018).</li><li>Kim, C. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MSC-Encapsulating+in+situ+Cross-Linkable+Gelatin+Hydrogels+To+Promote+Myocardial+Repair.">MSC-Encapsulating in situ Cross-Linkable Gelatin Hydrogels To Promote Myocardial Repair.</a> ACS Applied Bio Materials. 3 (3), 1646-1655 (2020).</li><li>Meirelles Lda, S., Nardi, N. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+marrow-derived+mesenchymal+stem+cell:+isolation,+in+vitro+expansion,+and+characterization.">Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization.</a> Br J Haematol. 123 (4), 702-711 (2003).</li><li>Ojha, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+the+structural+and+functional+changes+in+the+myocardium+following+focal+ischemia-reperfusion+injury.">Characterization of the structural and functional changes in the myocardium following focal ischemia-reperfusion injury.</a> Am J Physiol Heart Circ Physiol. 294 (6), 2435-2443 (2008).</li><li>Takagawa, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+infarct+size+measurement+in+the+mouse+chronic+infarction+model:+comparison+of+area-+and+length-based+approaches.">Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches.</a> Journal of Applied Physiology. 102 (6), 2104-2111 (2007).</li><li>Terrovitis, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+Quantification+and+Optimization+of+Acute+Cell+Retention+by+In+vivo+Positron+Emission+Tomography+After+Intramyocardial+Cardiac-Derived+Stem+Cell+Delivery.">Noninvasive Quantification and Optimization of Acute Cell Retention by In vivo Positron Emission Tomography After Intramyocardial Cardiac-Derived Stem Cell Delivery.</a> Journal of the American College of Cardiology. 54 (17), 1619-1626 (2009).</li><li>Dib, N., Khawaja, H., Varner, S., McCarthy, M., Campbell, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+Therapy+for+Cardiovascular+Disease:+A+Comparison+of+Methods+of+Delivery.">Cell Therapy for Cardiovascular Disease: A Comparison of Methods of Delivery.</a> Journal of Cardiovascular Translational Research. 4 (2), 177-181 (2011).</li></ol>

Gli idrogel GH iniettabili hanno un grande potenziale per applicazioni in vivo grazie alla loro capacità di incorporare omogeneamente diversi agenti terapeutici in situ. Inoltre, le loro proprietà fisiche e biochimiche possono essere facilmente manipolate in base a requisiti dipendenti dalla malattia. A questo proposito, sono stati proposti idrogel iniettabili per affrontare le principali limitazioni dell'attuale terapia con cellule staminali cardiache ostacolate dalla scarsa sopravvivenza e ritenzione cellulare (cioè...

La terapia con cellule staminali cardiache è emersa come un potenziale approccio per la riparazione e la rigenerazione delmiocardio 1,2. Nonostante i recenti risultati positivi nei modelli animali e negli studi clinici, l'applicazione della terapia a base di cellule staminali per la riparazione del miocardio è limitata a causa della bassa ritenzione e della scarsa sopravvivenza delle cellule iniettate nei tessuti cardiaci infarti3,4. Di conseguenza, l'uso dell'ingegneria tissutale a base cellulare, compresi i biomateriali iniettabili5,le chiazze cardiache6e ifogli cellulari 7,è stato intensamente studiato per migliorare la ritenzione cellulare e l'integrazione all'interno del miocardio ospite.
Tra i vari approcci potenziali alla riparazione del tessuto cardiaco bioingegnerato, gli idrogel iniettabili combinati con tipi di cellule appropriati, come le cellule staminali mesenchimali (MCC), le cellule staminali embrionali (ESC) e le cellule staminali pluripotenti indotte (IPSC), sono un'opzione interessante per fornire efficacemente le cellule nelle regioni del miocardio8,9. La gelatina, un noto polimero naturale, può essere utilizzata come matrice iniettabile grazie alla sua grande biocompatibilità, alla notevole biodegradabilità e alla ridotta immunogenicità rispetto a una vasta gamma di biomateriali utilizzati in applicazioni biomediche. Sebbene le piattaforme iniettabili a base di gelatina abbiano un grande potenziale, la loro applicabilità in vivo rimane limitata in base alla loro bassa rigidità meccanica e alla facile degradabilità nell'ambiente fisiologico.
Per superare questi limiti, è stato proposto un nuovo e semplice design di idrogel a base di gelatina costituiti da acido propionico idrossifenile per applicazioni in vivo. I coniugati gelatino-idrossifenil propionico (GH) possono essere reticolati in situ in presenza di un enzima, la perossidasi di rafano (HRP), e successivamente incapsulare vari farmaci, biomolecole o cellule all'interno dell'idrogel, suggerendo un grande potenziale nelle applicazioni di ingegneriatissutale 10,11,12,13,14. Inoltre, abbiamo recentemente studiato gli effetti terapeutici degli idrogel gh contenenti MSC incapsulati e dimostrato il loro uso nella riparazione e rigenerazione cardiaca di successo dopo l'MI in un modello murino15. In questo protocollo, descriviamo una semplice tecnica per l'incapsulamento e la proliferazione tridimensionale in vitro (3D) dei CCI all'interno degli idrogel GH. Introduciamo anche una procedura chirurgica progettata per generare un modello MI murino attraverso la legatura dell'arteria coronarica e il trapianto intramiocardico di idrogel GH a caricamento MSC nel cuore infarto.

<table>
	<tbody>
		<tr>
			<td>4 % paraformaldehyde (PFA)</td>
			<td>Intron</td>
			<td>IBS-BP031-2</td>
			<td></td>
		</tr>
		<tr>
			<td>5-0 silk suture</td>
			<td>AILEE</td>
			<td>SK534</td>
			<td></td>
		</tr>
		<tr>
			<td>8-0 polypropylene suture</td>
			<td>ETHICON</td>
			<td>M8732H</td>
			<td></td>
		</tr>
		<tr>
			<td>8-well chamber slide</td>
			<td>Nunc LAB-TEK</td>
			<td>154534</td>
			<td></td>
		</tr>
		<tr>
			<td>Angiocath Plus (22GA) catheter</td>
			<td>BD Angiocath Plus</td>
			<td>REF382423</td>
			<td></td>
		</tr>
		<tr>
			<td>Antibiotic-antimyocotic</td>
			<td>Gibco</td>
			<td>15240-062</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge</td>
			<td>GYROGEN</td>
			<td>1582MGR</td>
			<td></td>
		</tr>
		<tr>
			<td>Confocal microscope</td>
			<td>Zeiss</td>
			<td>LSM 510</td>
			<td></td>
		</tr>
		<tr>
			<td>Cover slipe</td>
			<td>MARIENFELD</td>
			<td>101242</td>
			<td></td>
		</tr>
		<tr>
			<td>Deluxe High Temperature Cautery kit</td>
			<td>Bovie</td>
			<td>QTY1</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Gibco</td>
			<td>11995-065</td>
			<td></td>
		</tr>
		<tr>
			<td>DPBS</td>
			<td>Gibco</td>
			<td>14040-133</td>
			<td></td>
		</tr>
		<tr>
			<td>Dual-syringe</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>EOSIN</td>
			<td>SIGMA-ALDRICH</td>
			<td>HT110116</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>EMSURE</td>
			<td>K49350783 739</td>
			<td></td>
		</tr>
		<tr>
			<td>FBS</td>
			<td>Gibco</td>
			<td>16000-044</td>
			<td></td>
		</tr>
		<tr>
			<td>Fechtner conjunctiva forceps titanium</td>
			<td>WORLD PRECISISON INSTRUMENTS</td>
			<td>WP1820</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorescein isothiocyanate isomer I (FITC)</td>
			<td>SIGMA-ALDRICH</td>
			<td>F7250</td>
			<td></td>
		</tr>
		<tr>
			<td>Forcep</td>
			<td>HEBU</td>
			<td>HB0458</td>
			<td></td>
		</tr>
		<tr>
			<td>Hair removal cream</td>
			<td>Ildong Pharmaceutical</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Heating pad</td>
			<td>Stoelting</td>
			<td>50300</td>
			<td>Homeothermic Blanket System</td>
		</tr>
		<tr>
			<td></td>
			<td></td>
			<td>50301</td>
			<td>Replacement Heating Pad for 50300 (10 X 12.5cm)</td>
		</tr>
		<tr>
			<td>Hematoxylin</td>
			<td>SIGMA-ALDRICH</td>
			<td>HHS80</td>
			<td></td>
		</tr>
		<tr>
			<td>Horseradish peroxide (HRP; 250-330 U/mg)</td>
			<td>SIGMA-ALDRICH</td>
			<td>P8375</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrogen peroxide (H2O2; 30 wt % in H2O)</td>
			<td>SIGMA-ALDRICH</td>
			<td>216763</td>
			<td></td>
		</tr>
		<tr>
			<td>Iodine</td>
			<td>Green Pharmaceutical</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>LIVE/DEAD cell staining kit</td>
			<td>Thermo Fisher</td>
			<td>R37601</td>
			<td></td>
		</tr>
		<tr>
			<td>Mechanical ventilator</td>
			<td>Harvard Apparatus</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Micro centrifuge</td>
			<td>HANIL</td>
			<td>Micro 12</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro needle holder</td>
			<td>KASCO</td>
			<td>37-1452</td>
			<td></td>
		</tr>
		<tr>
			<td>Micro scissor</td>
			<td>HEBU</td>
			<td>HB7381</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscope</td>
			<td>OLYMPUS</td>
			<td>SZ61</td>
			<td></td>
		</tr>
		<tr>
			<td>MT staining kit</td>
			<td>SIGMA-ALDRICH</td>
			<td>HT1079-1SET</td>
			<td>Weigert&#8217;s iron hematoxylin solution</td>
		</tr>
		<tr>
			<td></td>
			<td></td>
			<td>HT15-1KT</td>
			<td>Trichrome Stain (Masson) Kit</td>
		</tr>
		<tr>
			<td>Paraffin</td>
			<td>LK LABKOREA</td>
			<td>H06-660-107</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS buffer</td>
			<td>Gibco</td>
			<td>10010-023</td>
			<td></td>
		</tr>
		<tr>
			<td>PHK26 staining kit</td>
			<td>SIGMA-ALDRICH</td>
			<td>MINI26</td>
			<td></td>
		</tr>
		<tr>
			<td>Slide scanner</td>
			<td>Leica</td>
			<td>SCN400</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical scissor</td>
			<td>HEBU</td>
			<td>HB7454</td>
			<td></td>
		</tr>
		<tr>
			<td>Surgical tape</td>
			<td>3M micopore</td>
			<td>1530-1</td>
			<td></td>
		</tr>
		<tr>
			<td>Tissue cassette</td>
			<td>Scilab Korea</td>
			<td>Cas3003</td>
			<td></td>
		</tr>
		<tr>
			<td>Transducer gel</td>
			<td>SUNGHEUNG</td>
			<td>SH102</td>
			<td></td>
		</tr>
		<tr>
			<td>Trout-Barraquer needle holder curved</td>
			<td>KASCO</td>
			<td>50-3710c</td>
			<td></td>
		</tr>
		<tr>
			<td>Ultrasound system</td>
			<td>Philips</td>
			<td>Affiniti 50</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylene</td>
			<td>JUNSEI</td>
			<td>25175-0430</td>
			<td></td>
		</tr>
	</tbody>
</table>

intramyocardial transplantation of msc-loading injectable hydrogels after myocardial infarction in a murine model

Uno dei principali problemi che devono affrontare le attuali terapie con cellule staminali cardiache per prevenire l'insufficienza cardiaca postinfarct è il basso tasso di ritenzione e sopravvivenza delle cellule trapiantate all'interno del miocardio ferito, limitandone l'efficacia terapeutica. Recentemente, l'uso di biomateriali impalcatura ha attirato l'attenzione per migliorare e massimizzare la terapia con cellule staminali. L'obiettivo di questo protocollo è introdurre una tecnica semplice e diretta per trapiantare le cellule staminali mesenchimali derivate dal midollo osseo (MSC) utilizzando idrogel di acido propionico idrossifenile iniettabile (GH); gli idrogel sono favorevoli come piattaforma di somministrazione cellulare per applicazioni di ingegneria dei tessuti cardiaci a causa della loro capacità di essere collegati in situ incrociati e dell'elevata biocompatibilità. Presentiamo un metodo semplice per fabbricare idrogel GH a caricamento MSC (MSC/idrogel) e valutarne la sopravvivenza e la proliferazione in coltura tridimensionale (3D) in vitro. Inoltre, dimostriamo una tecnica per il trapianto intramiocardico di MSC / idrogel nei topi, descrivendo una procedura chirurgica per indurre l'infarto miocardico (MI) tramite la legatura coronarica discendente anteriore sinistro (LAD) e il successivo trapianto MSC / idrogels.

Per fornire efficacemente i CFC al miocardio infartuato, in questo protocollo sono stati utilizzati idrogel collegabili incrociati in situ a caricamento MSC descritti nella figura 1. Prima del trapianto in vivo, la proliferazione e la sopravvivenza dei MBC negli idrogel gh sono stati confermati da un saggio di colorazione in cellule vive / morte in vitro 3D (vivo: verde; morto: rosso). Come mostrato nella figura 2, le immagini rappresentative mostravano una suff...

Watch this Scientific Journal Video about Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model at JoVE.com

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

La terapia a base di cellule staminali è emersa come una strategia efficiente per riparare i tessuti cardiaci feriti dopo l'infarto del miocardio. Forniamo un'applicazione in vivo ottimale per il trapianto di cellule staminali utilizzando idrogel di gelatina che possono essere enzimaticamente incrociati.

Trapianto intramiocardico di idrogel iniettabili a carico MSC dopo infarto del miocardio in un modello murino

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Research

JoVE Journal

Medicine

Acute Myocardial Infarction in Rats

Infarto acuto del miocardio in topi

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

Ricerca

Medicina

Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction

Legatura dell&#39;arteria coronarica e di iniezione intramiocardico in un modello murino di infarto

Mouse models are a valuable tool for studying acute injury and chronic remodeling of the myocardium in vivo.  With the advent of genetic modifications to ...

Myocardial Infarction and Functional Outcome Assessment in Pigs

Infarto miocardico e funzionale Outcome Assessment in Pigs

Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One ...

A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.

Un idrogel Costruire e fibrina-based Glue approccio per fornire Therapeutics in una murino Myocardial Infarction Model.

The murine MI model is widely recognized in the field of cardiovascular disease, and has consistently been used as a first step to test the efficacy of ...

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

La valutazione dell&#39;outcome primario in un modello suino di infarto miocardico acuto

Mortality after acute myocardial infarction remains substantial and is associated with significant morbidity, like heart failure. Novel therapeutics are ...

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Infarto del miocardio in topi neonati, un modello di rigenerazione cardiaca

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murino discendente anteriore sinistra (LAD) Coronary legatura dell&#39;arteria: una migliore e semplificata del modello di infarto miocardico

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Modello di infarto miocardico Murine utilizzando la legatura permanente dell'arteria coronaria discendente anteriore sinistra

Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine ...

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

Un modello di criolesionismo per studiare l'infarto miocardico nel mouse

The use of animal models is essential for developing new therapeutic strategies for acute coronary syndrome and its complications. In this article, we ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Consegna intramiocardica ritardata di cellule staminali dopo l'infortunio di ricottamento ischemia in un modello Murine

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...