Protocole d’immunohistochimie et de distribution in situ de l’ARN au sein de l’embryon précoce de drosophile
May 6th, 2022
•Wei Zhang1*, Xinjuan Lei1*, Xin Zhou2,3*, Boling He1, Liqin Xiao1, Huimin Yue1, Shulin Wang1, Yuting Sun1, Yajun Wu1, Liyang Wang1,4, George Ghartey-Kwansah1, Odell D. Jones5, Joseph L. Bryant6, MengMeng Xu7, Jianjie Ma3, Xuehon Xu1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
Ici, nous décrivons un protocole pour la détection et la localisation de la protéine embryonnaire et de l’ARN de la drosophile , de la collecte à la pré-intégration et à l’intégration, à l’immunocoloration et à l’hybridation in situ de l’ARNm.
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