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DOI :
10.3791/61783-v
January 22nd, 2021
Chapters
0:04
Introduction
1:05
Spheroid Culture Preparation
1:47
Spheroid Generation
3:24
Spheroid Harvest
4:30
Results: Representative Colon Cancer Stem Cell Spheroid Formation and Analysis
6:06
Conclusion
このプロトコルは、Caco2結腸腺癌細胞から3次元スフェロイドを生成し、増殖させる新規、堅牢、再現性培養システムを提示する。この結果は、化学療法への応答を含む癌幹細胞生物学を研究するためのこのアプローチの適切性に関する最初の概念実証を提供する。
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