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DOI :
10.3791/61787-v
•
5:33 min
November 9th, 2020
Chapters
0:04
Introduction
0:50
Endpoint Degradation Studies with HiBiT CRISPR Target Proteins in Lytic Format with Optional Cell Viability Fluorescence Analysis
3:07
Results: Degradation Compound Analysis in a HiBiT CRISPR Cell Line
4:51
Conclusion
该协议描述了活细胞中蛋白质降解动力学的定量发光检测,这些活细胞已使用CRISPR / Cas9进行工程设计,以表达融合到目标蛋白质的无抗体内源性蛋白质检测标签。包括计算和获取定量降解参数、速率、Dmax、DC 50 和 Dmax50 的详细说明。
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