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DOI :
10.3791/61800-v
•
9:59 min
October 7th, 2020
Chapters
0:04
Introduction
0:51
Testis Dissection and Preparation
2:10
Transillumination-Assisted Microdissection
2:47
Squash Preparation and Stage Verification
4:04
Whole-Mount Seminiferous Tubule Immunostaining
7:04
Results: Representative Mouse Seminiferous Epithelial Cycle Staging
9:16
Conclusion
本プロトコルは、半日本円上皮周期の特定段階を表す成体マウス半細管の断面微小切除法、およびその中の細胞タイプ、および後にスカッシュ製剤および無傷の管状セグメントの免疫染色を記述する。
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