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DOI :
10.3791/61807-v
•
10:43 min
September 18th, 2020
Chapters
0:04
Introduction
0:44
Peripheral Blood Mononuclear Cell (PBMC) Isolation
1:40
Monocyte-Derived Cell Differentiation and Polarization
3:25
Mycobacterium tuberculosis (Mtb) Culture
5:00
Monocyte-Derived Cell Mtb Infection
6:03
Flow Cytometry Staining and Analysis
7:40
Results: Representative Phenotypic M1 and M2 Cell Characterization Before and After Mtb Infection
9:47
Conclusion
该协议提供了一种方法来研究人类M1或M2极化巨噬细胞中的 分枝杆菌 感染,该方法基于外周血单核细胞与感染GFP标记的病毒株H37Rv的巨噬细胞的分化,并使用10色面板(包括选定的M1/M2标记的表达)用流动细胞学进行分析。
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