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DOI :
10.3791/61807-v
•
10:43 min
September 18th, 2020
Chapters
0:04
Introduction
0:44
Peripheral Blood Mononuclear Cell (PBMC) Isolation
1:40
Monocyte-Derived Cell Differentiation and Polarization
3:25
Mycobacterium tuberculosis (Mtb) Culture
5:00
Monocyte-Derived Cell Mtb Infection
6:03
Flow Cytometry Staining and Analysis
7:40
Results: Representative Phenotypic M1 and M2 Cell Characterization Before and After Mtb Infection
9:47
Conclusion
このプロトコルは、ヒトM1-またはM2偏光マクロファージにおける 結核 菌感染を、GFP標識型毒性株H37Rvに感染したマクロファージ様細胞への末梢血単球の分化に基づいて研究し、M1/M2マーカーの選択を含む10色のパネルを使用してフローサイトメトリーで分析する方法を提供する。
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