Name: experimental autoimmune uveitis: an intraocular inflammatory mouse model
Uploaded: 2022-01-12T12:30:00
Description: Watch this Scientific Journal Video about Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model at JoVE.com

JG获得了UCL影响力学生奖学金和玫瑰树信托基金的资金，以支持CB。VC获得了Akari Therapeutics Inc.的研究合作资助。我们要感谢伦敦大学学院眼科研究所，生物服务部门，特别是Alison O'Hara女士和她的团队的技术支持。

所有实验均按照1986年英国动物（科学程序）法案和机构动物福利和伦理审查机构（AWERB）指南进行。
1. 外壳 C57BL/6J 小鼠
<ol><li>将小鼠置于特定的无病原体环境中，在12小时的明暗循环中 随意提供食物和水。</li>
	<li>对成年女性C57BL / 6J进行所有实验（优先考虑女性，因为葡萄膜炎患者中女性与男性的发病率为1.4：1）。按体重和年龄随机分配6-8周龄之间的雌性C57BL / 6J小...

<ol>
	<li>Lyu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TMP778,+a+selective+inhibitor+of+RORgammat,+suppresses+experimental+autoimmune+uveitis+development,+but+affects+both+Th17+and+Th1+cell+populations.">TMP778, a selective inhibitor of RORgammat, suppresses experimental autoimmune uveitis development, but affects both Th17 and Th1 cell populations.</a> The European Journal of Immunology. 48, 1810-1816 (2018).</li><li>Klaska, I. P., Forrester, J. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+autoimmune+uveitis.">Mouse models of autoimmune uveitis.</a> Current Pharmaceutical Design. 21, 2453-2467 (2015).</li><li>Eskandarpour, M., Alexander, R., Adamson, P., Calder, V. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pharmacological+Inhibition+of+Bromodomain+Proteins+Suppresses+Retinal+Inflammatory+Disease+and+Downregulates+Retinal+Th17+Cells.">Pharmacological Inhibition of Bromodomain Proteins Suppresses Retinal Inflammatory Disease and Downregulates Retinal Th17 Cells.</a> The Journal of Immunology. 198, 1093-1103 (2017).</li><li>Mochizuki, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preclinical+and+clinical+study+of+FK506+in+uveitis.">Preclinical and clinical study of FK506 in uveitis.</a> Current Eye Research. 11, 87-95 (1992).</li><li>Nguyen, Q. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravitreal+Sirolimus+for+the+Treatment+of+Noninfectious+Uveitis:+Evolution+through+Preclinical+and+Clinical+Studies.">Intravitreal Sirolimus for the Treatment of Noninfectious Uveitis: Evolution through Preclinical and Clinical Studies.</a> Ophthalmology. 125, 1984-1993 (2018).</li><li>Leal, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-TNF+Drugs+for+Chronic+Uveitis+in+Adults-A+Systematic+Review+and+Meta-Analysis+of+Randomized+Controlled+Trials.">Anti-TNF Drugs for Chronic Uveitis in Adults-A Systematic Review and Meta-Analysis of Randomized Controlled Trials.</a> Frontiers in Medicine (Lausanne). 6, 104 (2019).</li><li>Chen, Y. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functionally+distinct+IFN-?++IL-17A++Th+cells+in+experimental+autoimmune+uveitis:+T-cell+heterogeneity,+migration,+and+steroid+response.">Functionally distinct IFN-?+ IL-17A+ Th cells in experimental autoimmune uveitis: T-cell heterogeneity, migration, and steroid response.</a> European Journal of Immunology. 50 (12), 1941-1951 (2020).</li><li>Xu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+clinical+grading+system+for+retinal+inflammation+in+the+chronic+model+of+experimental+autoimmune+uveoretinitis+using+digital+fundus+images.">A clinical grading system for retinal inflammation in the chronic model of experimental autoimmune uveoretinitis using digital fundus images.</a> Experimental Eye Research. 87, 319-326 (2008).</li><li>Copland, D. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+clinical+time-course+of+experimental+autoimmune+uveoretinitis+using+topical+endoscopic+fundal+imaging+with+histologic+and+cellular+infiltrate+correlation.">The clinical time-course of experimental autoimmune uveoretinitis using topical endoscopic fundal imaging with histologic and cellular infiltrate correlation.</a> Investigative Ophthalmology &amp; Visual Science. 49, 5458-5465 (2008).</li><li>Dick, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Doyne+lecture+2016:+intraocular+health+and+the+many+faces+of+inflammation.">Doyne lecture 2016: intraocular health and the many faces of inflammation.</a> Eye (Lond). 31, 87-96 (2017).</li><li>Agarwal, R. K., Silver, P. B., Caspi, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rodent+models+of+experimental+autoimmune+uveitis.">Rodent models of experimental autoimmune uveitis.</a> Methods in Molecular Biology. 900, 443-469 (2012).</li><li>Caspi, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+look+at+autoimmunity+and+inflammation+in+the+eye.">A look at autoimmunity and inflammation in the eye.</a> Journal of Clininical Investigation. 120, 3073-3083 (2010).</li><li>Caspi, R. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+experimental+autoimmune+uveitis.">Mouse models of experimental autoimmune uveitis.</a> Ophthalmic Research. 40, 169-174 (2008).</li><li>Shao, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Severe+chronic+experimental+autoimmune+uveitis+(EAU)+of+the+C57BL/6+mouse+induced+by+adoptive+transfer+of+IRBP1-20-specific+T+cells.">Severe chronic experimental autoimmune uveitis (EAU) of the C57BL/6 mouse induced by adoptive transfer of IRBP1-20-specific T cells.</a> Experimental Eye Research. 82, 323-331 (2006).</li><li>Horai, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbiota-Dependent+Activation+of+an+Autoreactive+T+Cell+Receptor+Provokes+Autoimmunity+in+an+Immunologically+Privileged+Site.">Microbiota-Dependent Activation of an Autoreactive T Cell Receptor Provokes Autoimmunity in an Immunologically Privileged Site.</a> Immunity. 43, 343-353 (2015).</li><li>Chen, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparative+analysis+of+induced+vs.+spontaneous+models+of+autoimmune+uveitis+targeting+the+interphotoreceptor+retinoid+binding+protein.">Comparative analysis of induced vs. spontaneous models of autoimmune uveitis targeting the interphotoreceptor retinoid binding protein.</a> PLoS One. 8, 72161 (2013).</li><li>Chen, J., Qian, H., Horai, R., Chan, C. C., Caspi, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+optical+coherence+tomography+and+electroretinography+to+evaluate+retinal+pathology+in+a+mouse+model+of+autoimmune+uveitis.">Use of optical coherence tomography and electroretinography to evaluate retinal pathology in a mouse model of autoimmune uveitis.</a> PLoS One. 8, 63904 (2013).</li><li>Harry, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+of+autoimmune+retinal+disease+by+lovastatin+does+not+require+Th2+cytokine+induction.">Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.</a> Journal of Immunology. 174, 2327-2335 (2005).</li></ol>

实验动物模型是研究疾病发病机制和新治疗范式临床前测试的必要工具。在当前的协议中，我们讨论了诱导，监测和评分EAU的方法，这是眼内炎症性葡萄膜炎的实验模型。当根据此处概述的方案执行所有程序时，该EAU模型具有超过95%的疾病发病率，并导致慢性单相EAU的发展。为了达到这一发生率水平，我们强调抗原制备和乳剂注射的重要性，这两者都在上面详细介绍。EAU在动物中的主要特征是视?...

该协议将演示如何通过在乳化佐剂中单次皮下注射视网膜抗原来诱导C57BL / 6J小鼠中的实验性自身免疫性葡萄膜炎（EAU）。监测和评估疾病进展的方法将通过眼底成像和组织学检查进行详细说明，并在其中概述测量参数。此外，还将讨论荧光素血管造影，这是一种检查视网膜血管结构和通透性的技术。
该EAU模型概括了人类非感染性后葡萄膜炎在临床病理学特征以及驱动疾病的基本细胞和分子机制方面的核心特征。EAU由自反应CD4 + T淋巴细胞的Th1和/或Th17亚群介导，如过继转移实验和IFNγ耗尽小鼠所示1。我们对这些细胞在葡萄膜炎中潜在作用的大部分理解来自研究EAU2，其中在视网膜组织内检测到Th1和Th17细胞3。通常，EAU被用作临床前模型来评估新疗法在减轻疾病方面的效用。成功调节EAU疾病的治疗方法在临床上显示出一定的疗效，并达到了FDA批准的地位。例如一组免疫调节药物，例如T细胞靶向疗法：环孢素，FK-506和雷帕霉素4，5，6。最近，在该模型中还探索了针对新途径的干预措施，以研究对疾病结果的机制和影响。这些包括通过染色质读取器Bromodomain末端外（BET）蛋白和P-TEFb抑制剂靶向转录调节3。此外，更传统的方法（如VLA-4抑制剂）最近已证明通过调节效应CD4 + T细胞抑制EAU7。此外，还发现用 RORγt 反向激动剂 TMP778 靶向 Th17 细胞可显着抑制 EAU8。此外，该模型为研究视网膜中的慢性自身免疫性炎症以及伴随的潜在机制（如淋巴细胞启动）提供了机会。
EAU临床前研究的主要读数是通过进行视网膜眼底镜成像进行临床评估，较少通过光学相干断层扫描（OCT）评估视网膜完整性。然后在终止时通过流式细胞术进行视网膜组织病理学评估和视网膜细胞的免疫表型。眼底镜检查是一种易于使用的实时成像系统，可以对整个视网膜进行快速且可重复的临床评估。对于免疫组织化学评估，这些技术基于视网膜切片的制备，使我们能够研究炎症和结构损伤程度的组织结构9。该协议将概述所有所用技术的评估标准和常规评分系统。使用眼底镜成像记录的损伤程度通常与组织学变化密切相关。这种监测和评估疾病严重程度的双重方法提供了更高的灵敏度和更可靠的测量结果。
EAU是一种成熟的常用模型，用于免疫介导的眼病的临床前测试和调查。该模型可靠且可重复，疾病发病率>95%，并生成全面的数据，可用于验证或否定治疗眼内炎症性疾病的新疗法，这是全球工作年龄失明的主要原因10。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>antisedan</td>
			<td>ZOETIS, USA</td>
			<td></td>
			<td>for waking up</td>
		</tr>
		<tr>
			<td>Complete Freund&#8217;s Adjuvant; CFA</td>
			<td>Sigma, UK</td>
			<td>F5881</td>
			<td>for immunisation&#160;</td>
		</tr>
		<tr>
			<td>Domitor</td>
			<td>Orion Pharma, Finland</td>
			<td></td>
			<td>for anesthesia</td>
		</tr>
		<tr>
			<td>Flourescein</td>
			<td>Sigma, UK</td>
			<td>F2456</td>
			<td>for Angiography</td>
		</tr>
		<tr>
			<td>IRBP1-20</td>
			<td>Chamberidge peptide, UK</td>
			<td></td>
			<td>peptide;antigen&#160;</td>
		</tr>
		<tr>
			<td>Ketamine</td>
			<td>Orion Pharma, Finland</td>
			<td></td>
			<td>for anesthesia</td>
		</tr>
		<tr>
			<td>Micron III</td>
			<td>Phoenix Research, USA</td>
			<td></td>
			<td>for fundoscopy</td>
		</tr>
		<tr>
			<td>Mouse Serum</td>
			<td>Sigma, UK</td>
			<td>M5905</td>
			<td>for immunisation&#160;</td>
		</tr>
		<tr>
			<td>Mycobacterium terberculosis</td>
			<td>Sigma, UK</td>
			<td>344289</td>
			<td>for immunisation&#160;</td>
		</tr>
		<tr>
			<td>Pertussis Toxin</td>
			<td>Sigma, UK</td>
			<td>P2980</td>
			<td>for immunisation&#160;</td>
		</tr>
		<tr>
			<td>phenylephrine hydrochloride 2.5%&#160;</td>
			<td>Bausch &#38; Lomb UK&#160;</td>
			<td>PHEN25</td>
			<td>for dilation&#160;</td>
		</tr>
		<tr>
			<td>Tropicamide 1%</td>
			<td>SANDOZ</td>
			<td></td>
			<td>for dilation&#160;</td>
		</tr>
		<tr>
			<td>Viscotears</td>
			<td>WELDRICKS Pharmacy, UK</td>
			<td>2082642</td>
			<td>for eye lubrication</td>
		</tr>
	</tbody>
</table>

experimental autoimmune uveitis: an intraocular inflammatory mouse model

实验性自身免疫性葡萄膜炎（EAU）是由免疫细胞对自身抗原的反应驱动的。这种非感染性眼内炎症性疾病模型的许多特征概括了影响人类的后葡萄膜炎的临床表型。EAU已被可靠地用于研究新型炎症疗法的疗效，其作用方式，并进一步研究支持眼内疾病疾病进展的机制。在这里，我们提供了关于C57BL / 6J小鼠EAU诱导的详细方案 - 这是使用最广泛的具有这种疾病易感性的模式生物。疾病严重程度和进展的临床评估将通过眼底镜检查、组织学检查和荧光素血管造影进行。诱导程序包括皮下注射含有来自眼蛋白间光感受器类视黄醇结合蛋白（也称为视黄醇结合蛋白 3）的肽 （IRBP1-20）、完全弗氏佐剂 （CFA） 的乳剂，并补充杀死 结核分枝杆菌。 在颈部后部注射这种粘性乳剂，然后腹膜内注射 百日咳博德特 氏菌毒素。在症状发作（第12-14天）和全身麻醉下，拍摄眼底镜图像以通过临床检查评估疾病进展。这些数据可以直接与较晚的时间点和疾病高峰期（第20-22天）的数据进行比较，并分析差异。同时，该协议允许研究人员使用荧光素血管造影评估血管通透性和损伤的潜在差异。EAU可以在其他小鼠品系中诱导 - 野生型或转基因 - 并与新疗法相结合，为研究药物疗效和/或疾病机制提供灵活性。

在该协议中，我们描述了一种通过用源自IRBP的葡萄膜肽免疫小鼠来诱导实验性自身免疫性葡萄膜炎（EAU）模型的分步方法。涵盖了采用广泛使用和易于获取的方法评估疾病，尽管这些方法不是排他性的，可以添加到其他成像技术中或部分替代。C57BL / 6J小鼠中EAU的最初迹象可以在免疫后两周内检测到，并在三周内达到疾病高峰，如图 1所示。眼底镜下的变化在疾病进展期间被...

Watch this Scientific Journal Video about Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model at JoVE.com

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

在本报告中，我们提出了一种协议，允许研究人员生成眼内葡萄膜炎的小鼠模型。通常被称为实验性自身免疫性葡萄膜炎（EAU），这个已建立的模型捕获了人类疾病的许多方面。在这里，我们将描述如何使用几个读数来诱导和监测疾病进展。

实验性自身免疫性葡萄膜炎：眼内炎症小鼠模型

Experimental Autoimmune Uveitis (EAU) is driven by immune cells responding to self-antigens. Many features of this non-infectious, intraocular ...

该模型可用于研究涉及免疫介导的人后葡萄膜炎发病机制的细胞机制。该技术的意义延伸到人类葡萄膜炎的转化研究，以监测新型药物疗效并提供化学疾病进展的机制见解。使用该模型的主要优点是疾病诱导的可靠性以及使用非侵入性内窥镜技术，使我们能够监测疾病进展。在这里，我们描述了如何使用几个读数来诱导疾病和评估病理为了制备IRBP 1-20完整的弗氏佐剂或CFA，将计算量的肽重悬于最小体积为100%DMSO。当粉末完全溶解后，向管中加入少量PBS，直到根据要注射的小鼠数量使用温和搅拌混合溶液达到最终计算的PBS体积。制备完整的弗氏佐剂后，轻轻且频繁地移液以形成粘稠且均匀分布的乳液，同时以一比一的比例滴加DMSO PBS肽溶液与悬浮液。要给溶液充气，请使用设置为700微升的1000微升移液器重复移液，直到获得浓稠的奶油乳液。在注射肽悬液之前，将每只待注射的小鼠放入单独的笼子中，并使用配备23号针头的1毫升注射器腹膜内向每只动物提供1.5微克百日咳博德特氏菌毒素在100微升RPMI 1640培养基中补充有1%小鼠血清。注射百日咳毒素溶液后，应将小鼠保持在肋骨状位置，并将皮肤捏紧，在颈部后部形成帐篷状结构。将针头穿到手指和拇指之间的空间，将200微升IRBP乳液注入帐篷皮肤，注射后保持压力，旋转针头闭合皮肤后回缩。为了对临床疾病进行评分，在注射后第21天，确认注射麻醉肽的小鼠对花瓣反射缺乏反应，并将动物限制在scruff中。注射后立即将1%托吡卡胺和2%去氧肾上腺素局部涂抹在每只眼睛的角膜上。当瞳孔完全散大后，将鼠标放在专用的显微镜载物台上。放置显微镜以完全进入视网膜。在整个成像过程中根据需要调整目镜，以查看视盘和视网膜，并获取整个视网膜区域的图像，调整并覆盖外围的所有角落。为了测量血管泄漏，在颈部后部皮下注射100微升2%荧光素，并重新定位小鼠，使视网膜位于实时图像的中间。将眼底镜置于465至490纳米的蓝光激发滤光片上，然后在荧光注射后1.5和7分钟获取每个图像。获取所有图像后，腹膜内注射抗镇静剂以进行恢复，并将小鼠放在预热垫上的笼子中，通过监测获得湿浸泡的饮食，直到小鼠恢复意识。对于图像的临床疾病评分，临床评估基于视盘炎症、视网膜血管袖带、视网膜组织浸润和结构损伤的严重程度。以 1 到 5 的等级对这些参数中的每一个进行评分。总分代表全眼临床疾病，每只眼睛最高可得20分。在对研究的最终临床终点进行眼底成像后，对小鼠实施安乐死。对于组织学分析，将眼睑分开以进入整个眼睛，然后将弯曲的镊子放在眼球后面以抓住眼眶结缔组织和视神经并去除眼睛。将去核眼放入一到五毫升的4%戊二醛中至少15分钟，然后将器官转移到一到五毫升的10%甲醛中至少24小时。组织学组织染色后，根据标准方案，根据免疫细胞浸润水平和视网膜损伤程度，以0到3的等级分配分数。在疾病进展期间，眼底镜下的变化分为炎症变化，包括视网膜组织、血管和视盘炎症以及视网膜结构损伤，以及基于浸润免疫细胞和结构损伤的组织学变化。可以对这些临床和组织病理学变化进行分级和评分，以评估疾病进展和评估治疗干预效果。血管渗漏也是该模型和人类葡萄膜炎的病理特征。如本分析所示，荧光素可用于可视化血管渗漏，作为该模型的另一个读数。对于研究人员来说，确保正确制备乳液时没有溶液之间分离的迹象至关重要。它们应充分混合，最终产品质地应光滑。该程序可以与其他药物给药方法和用于表型浸润视网膜免疫细胞群的细胞术结合使用。

Research

JoVE Journal

Immunology and Infection

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

实验转移和CTL过继转移免疫小鼠模型

Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. The tumor cells are injected intravenously (i.v) into ...

科研

免疫与感染

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis

鼠标垂体免疫原的制备诱导实验性自身免疫性垂体炎

Autoimmune hypophysitis is a chronic inflammation of the pituitary gland caused or accompanied by autoimmunity1.  It has traditionally been considered a ...

Induction of Experimental Autoimmune Hypophysitis in SJL Mice

SJL小鼠诱导实验性自身免疫性垂体炎

Autoimmune hypophysitis can be reproduced experimentally by the injection of pituitary proteins mixed with an adjuvant into susceptible mice1. Mouse ...

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis EAE Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS) and has been used as an animal model for ...

Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice

Myelin Oligodendrocyte Glycoprotein MOG35-55 Induced Experimental Autoimmune Encephalomyelitis EAE in C57BL/6 Mice

髓鞘少突胶质细胞糖蛋白（MOG<sub&gt; 35-55</sub&gt;）诱导实验性自身免疫性脑脊髓炎（EAE）的C57BL / 6小鼠

Multiple sclerosis is a chronic neuroinflammatory demyelinating disorder of the central nervous system with a strong neurodegenerative component. While ...

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

从小鼠皮肤使用酶消化和梯度分离提取白细胞浸润的

Dissociating murine skin into a single cell suspension is essential for downstream cellular analysis such as the characterization of infiltrating immune ...

Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies

简单而高效的生产和鼠标髓鞘少突胶质糖蛋白的纯化的实验性自身免疫性脑脊髓炎研究

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), thought to occur as a result of autoimmune responses ...

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

IL-8瞬时转化的小鼠模型<em&gt;体内</em&gt;长期监测炎症反应

Airway inflammation is often associated with bacterial infections and represents a major determinant of lung disease. The in vivo determination of the ...

Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model

味觉信号蛋白废除对炎症性肠病小鼠肠道炎症的影响

Inflammatory bowel disease (IBD) is one of the immune-related gastrointestinal disorders, including ulcerative colitis and Crohn's disease, that affects ...

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试

Type 1 Diabetes (T1D) is characterized by islet-specific autoimmunity leading to beta cell destruction and absolute loss of insulin production. In the ...