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DOI :
10.3791/61837-v
October 27th, 2020
Chapters
0:05
Introduction
0:34
Establishment of an Oxygen-Glucose Deprivation Model and Time-Temperature Protocol
2:30
CCK-8 Viability Assay and Analysis of Flow Cytometry for Apoptosis
3:59
Mitochondrial Depolarization Assessment and Reactive Oxygen Species Assay
5:11
Measurement of Caspase 3/ Caspase 8 Activity
5:51
Results: Evaluation of Cell Viability, Apoptosis, Mitochondrial Function, and Caspase Activity
7:40
Conclusion
心筋保護に対する低体温の異なる程度の明確な影響は十分に評価されていない。本研究の目的は、ヒト心筋細胞系モデルにおける異なる低体温症治療に続く細胞死のレベルを定量化し、将来の詳細な分子研究の基礎を築くことであった。
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