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DOI :
10.3791/61837-v
October 27th, 2020
Chapters
0:05
Introduction
0:34
Establishment of an Oxygen-Glucose Deprivation Model and Time-Temperature Protocol
2:30
CCK-8 Viability Assay and Analysis of Flow Cytometry for Apoptosis
3:59
Mitochondrial Depolarization Assessment and Reactive Oxygen Species Assay
5:11
Measurement of Caspase 3/ Caspase 8 Activity
5:51
Results: Evaluation of Cell Viability, Apoptosis, Mitochondrial Function, and Caspase Activity
7:40
Conclusion
심근 보호에 저체온증의 다른 정도의 뚜렷한 영향은 철저하게 평가되지 않았습니다. 본 연구의 목표는 인간 심근세포 기반 모델에서 다른 저체온증 치료에 따른 세포 사멸 의 수준을 정량화하여 미래의 심층 분자 연구의 토대를 마련하는 것이었습니다.
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