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DOI :
10.3791/61844-v
November 19th, 2020
Chapters
0:05
Introduction
0:45
S30 Ribosomal Protein Lysate Preparation
2:17
Membrane Scaffold Protein 1E3D1 (MSP1E3D1) Nanodisc Assembly
3:24
3-mL Continuous Exchange Cell-Free (CECF) Reaction Setup
4:10
60-Microliter CECF Reaction Setup
6:07
Results: Representative Preformed Nanodisc-Modified Membrane Protein Characterization
7:40
Conclusion
사전 형성된 나노디스크에 공동 번역하여 삽입하면 세제와의 접촉 없이 정의된 지질 환경에서 무세포 합성된 막 단백질을 연구할 수 있습니다. 이 프로토콜은 필수 시스템 구성 요소의 준비와 발현 효율성 및 샘플 품질을 개선하기 위한 중요한 파라미터를 설명합니다.
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