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DOI :
10.3791/61868-v
•
5:52 min
November 25th, 2020
Chapters
0:04
Introduction
1:07
Explant Preparation
3:46
Results: Analysis of Cell Types Found in Retinal Explant
4:49
Conclusion
该协议描述了小鼠 神经视网膜的细胞图外植,与视网膜色素上皮(RPE)一起在R16定义的介质中培养,不含血清和抗生素。与 体内 实验相比,这种方法性能相对简单、成本更低、耗时,可适应多种实验应用。
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