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DOI :
10.3791/61871-v
September 22nd, 2020
Chapters
0:04
Introduction
0:38
In Vitro Transcription
1:12
Translation Material Preparation
1:54
Liposome Preparation
3:07
In Vitro Translation
5:14
Proteoliposome Purification
5:56
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue (CBB) Staining
6:57
Results: Representative Proteoliposome Production by Bilayer-Dialysis
8:01
Conclusion
该协议描述了一种有效的无细胞方法,通过使用小麦无细胞系统和脂质体的双层透析方法生产高质量的蛋白水产体。该方法为膜蛋白的功能分析、药物靶点筛选和抗体开发提供了合适的手段。
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