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DOI :
10.3791/61871-v
September 22nd, 2020
Chapters
0:04
Introduction
0:38
In Vitro Transcription
1:12
Translation Material Preparation
1:54
Liposome Preparation
3:07
In Vitro Translation
5:14
Proteoliposome Purification
5:56
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue (CBB) Staining
6:57
Results: Representative Proteoliposome Production by Bilayer-Dialysis
8:01
Conclusion
このプロトコルは、小麦無細胞系およびリポソームを用いた二重層透析法による高品質のプロテオリポソームの効率的な無細胞生産法を記載する。この方法は、膜タンパク質の機能解析、創薬標的のスクリーニング、および抗体開発に適した手段を提供します。
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