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Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

5.0K Views

08:41 min

December 4th, 2020

December 4th, 2020

4,991 Views

0:04

Introduction

1:04

Nasonia Rearing

2:19

Alignment of White and Black Pupae

3:21

Needle Preparation

3:53

Adult Injection with Aspirator Tube

5:04

Adult Injection with Aspirator Tube

6:32

Post-Injection Care and Mutant Screening

7:27

Results: Survival and Efficiency Rate of RNAi and CRISPR/Cas9 via Pupal and Adult Injections

7:52

Conclusion

Transcript

Research on non-canonical insect model, like parasitoid wasp, is limited because embryonic microinjection is very challenging. Our method bypasses this problem, enabling site-specific and heritable germline mutations. Injecting pupae or adult fema

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Here, we describe methods for efficient pupal and adult injections in Nasonia vitripennis as accessible alternatives to embryo microinjection, enabling functional analysis of genes of interest using either RNA-silencing via RNA interference (RNAi) or gene knockout via CRISPR/Cas9 genome editing.

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